PXD067892

PXD067892 is an original dataset announced via ProteomeXchange.

Title	In situ Proteomics Unveils Specialized Domains for Extrasynaptic Signaling on Neuronal Cilia
Description	Neuronal cilia have emerged as signaling hubs, but their molecular composition and integration with synaptic communication remain unexplored. Using an engineered Arl13b-TurboID mouse model, we achieved robust cilia-specific biotinylation and proteomic profiling across diverse cell types. Comparative analyses revealed that brain and kidney cilia share only half of their proteome, with neuronal cilia uniquely enriched in synaptic proteins, adhesion molecules, and neurotransmitter receptors. Surprisingly, several signaling and adhesion molecules localize to neuronal cilia in discrete patterns maintained by active retrieval mechanisms. 16-fold expansion microscopy of mouse cortex revealed that the NMDA receptor is non-randomly positioned on ciliary membranes near glutamatergic synapses, suggesting that cilia eavesdrop on synaptic activity. These findings highlight the diversity and specialization of mammalian ciliary proteomes and indicates that nanoscale organization may endow neuronal cilia with extrasynaptic functions.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:10:45.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Hsiang Chang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-29 20:13:35	ID requested
⏵ 1	2026-06-07 16:10:46	announced

Chang CH, Trinh VN, Novak SW, Lokesh NR, Montecinos CK, Boassa D, Pownall ME, Kalocsay M, Nachury MV, In situ proteomics unveils specialized domains for extrasynaptic signaling on neuronal cilia. Sci Adv, 12(23):eaed5548(2026) [pubmed]

10.1126/sciadv.aed5548;

submitter keyword: brain,cilia, mouse, proteome

Maxence V. Nachury
contact affiliation	University of California, San Francisco
contact email	maxence.nachury@ucsf.edu
lab head
Chia-Hsiang Chang
contact affiliation	UCSF
contact email	chiaoceanblue@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067892

PRIDE project URI

• PRIDE
  1. PXD067892
     1. Label: PRIDE project
     2. Name: In situ Proteomics Unveils Specialized Domains for Extrasynaptic Signaling on Neuronal Cilia