PXD067890

PXD067890 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomic analysis of serum from patients with postherpetic neuralgia reveals a key role of Endoglin in neuropathic pain via TGF-β/Smad2 signaling
Description	This dataset comprises a Tandem Mass Tag (TMT)-based quantitative proteomic analysis of serum samples from patients with postherpetic neuralgia (PHN) compared to healthy control subjects. The study aimed to identify differentially expressed proteins involved in the pathogenesis of neuropathic pain. Peripheral blood serum was collected from three PHN patients and three age- and sex-matched healthy volunteers. Proteins were digested with trypsin, labeled with TMT isobaric tags, and fractionated by reversed-phase liquid chromatography. The peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a SCIEX TripleTOF 5600+ instrument. Our analysis identified 127 differentially expressed proteins (79 down-regulated and 48 up-regulated in the PHN group). Notably, Endoglin (CD105), a key endothelial cell membrane glycoprotein, was one of the significantly down-regulated proteins. This discovery was subsequently validated by ELISA in a larger cohort (n=30 per group). The proteomic profiling presented in this dataset led to the mechanistic hypothesis that reduced Endoglin expression contributes to neuropathic pain by disrupting the blood-spinal cord barrier (BSCB) integrity via inhibition of the TGF-β/Smad2 signaling pathway. This hypothesis was successfully confirmed in a spared nerve injury (SNI) rat model through behavioral, biochemical, and histological experiments, as detailed in our associated manuscript. This dataset provides the foundational proteomic evidence for the role of Endoglin in neuropathic pain and serves as a valuable resource for identifying potential biomarkers and therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:22:07.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhou FeiRan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-29 18:05:19	ID requested
⏵ 1	2025-10-05 16:22:08	announced

Liao S, Zhou FR, Li Q, Jin T, Xiang GF, Wang X, Liu Y, Zhu H, Liu Q, Liu Y, Zhang Y, /Smad2 signaling pathway. Front Mol Neurosci, 18():1603619(2025) [pubmed]

10.3389/fnmol.2025.1603619;

submitter keyword: Quantitative proteomics, Endoglin (CD105), Neuropathic pain, Tandem mass tag (TMT), TGF-β/Smad2 signaling,ostherpetic neuralgia (PHN), Biomarker, Blood-spinal cord barrier (BSCB)

Ying Zhang
contact affiliation	Department of Anesthesiology,The Affiliated Traditional Chinese Medicine Hospital , Southwest Medical University, Luzhou,646000,Sichuan,China
contact email	zhangying021210@163.com
lab head
Zhou FeiRan
contact affiliation	Department of Pain ,The Affiliated Traditional Chinese Medicine Hospital , Southwest Medical University, Luzhou,646000,Sichuan,China
contact email	zhoufeiran2023@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD067890

PRIDE project URI

• PRIDE
  1. PXD067890
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomic analysis of serum from patients with postherpetic neuralgia reveals a key role of Endoglin in neuropathic pain via TGF-β/Smad2 signaling