PXD067885

PXD067885 is an original dataset announced via ProteomeXchange.

Title	Plasma Proteome Stability at the Time of Sudden Cardiac Arrest: Implications for Biomarker Studies in Post-Resuscitation Survival
Description	Plasma serves as a crucial source of circulating protein biomarkers for advancing resuscitation strategies in sudden cardiac arrest (SCA). However, emergency blood collection under SCA introduces preanalytical variables that may affect proteomic analyses. This study assessed plasma protein stability under blood handling conditions that simulate real-world first responder scenarios. Blood from four individuals was aliquoted and subjected to different (a) initial storage conditions and (b) delays after collection, simulating first responder blood handling scenarios. Plasma was analyzed using data-independent acquisition mass spectrometry (DIA-MS) for global proteomics and parallel reaction monitoring (PRM-MS) for 16 SCA biomarker candidates. Global proteomic variation was primarily driven by inter-individual differences, with less influence from storage conditions or delays. Notably, 87.5% of the candidate biomarkers (14/16) remained stable under ideal storage (4°C storage immediately after blood collection), and 62.5% (10/16) retained stability even under worst-case (room temperature storage for 8 hours after blood collection) for up to 24 hours. However, several proteins lost stability with delayed processing, even under ideal storage. Our study highlights the critical importance of processing blood within 24 hours of collection and accounting for protein stability to preserve the reliability of biomarkers for improving resuscitation strategies in SCA.
HostingRepository	PanoramaPublic
AnnounceDate	2026-04-29
AnnouncementXML	Submission_2026-04-29_16:48:50.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jihyeon Lee
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(4); Label:13C(6)15N(2)
Instrument	Orbitrap Exploris 480; Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-29 13:18:36	ID requested
⏵ 1	2026-04-29 16:48:51	announced

Lee J, Nakamura K, Fu Q, Reinier K, Haghani A, Chugh H, Chugh SS, Van Eyk JE, Plasma Proteome Stability at the Time of Sudden Cardiac Arrest: Implications for Biomarker Studies in Post-Resuscitation Survival. J Proteome Res, 25(1):368-378(2026) [pubmed]

submitter keyword: blood collection, protein biomarkers, pre-analytical variables, mass spectrometry, sudden cardiac arrest

Jennifer Van Eyk
contact affiliation	Cedars Sinai Medical Center
contact email	jennifer.vaneyk@cshs.org
lab head
Jihyeon Lee
contact affiliation	Cedars Sinai Medical Center
contact email	jihyeon.lee@cshs.org
dataset submitter

Panorama Public dataset URI