PXD067881

PXD067881 is an original dataset announced via ProteomeXchange.

Title	Metalloproteome remodeling in calprotectin-stressed Acinetobacter baumannii using chemoproteomics
Description	The growth of bacterial pathogens is limited by nutritional immunity, where the infected host deploys metal scavenging proteins to starve the pathogen of essential transition metals. An important transition metal-sequestering protein is the Ca2+-activated S100A8-S100A9 heterotetramer, calprotectin (CP). Prior work reveals that CP induces a significant Fe- and Zn-starvation response in the Gram-negative opportunistic pathogen, Acinetobacter baumannii, in liquid culture. Here, we employ a quantitative thiol-specific chemoproteomics platform to pinpoint changes in abundance-corrected cysteine reactivity, and by extension cellular metal occupancy in metalloenzymes, that occur when A. baumannii is challenged with physiological CP in liquid culture relative to an untreated WT control. Changes in protein abundance with CP stress reveals a pronounced Zn-limitation and Fe-starvation response and reciprocal regulation of three enzymes of central carbon metabolism, including aconitase. A majority of 2645 quantifiable Cys-containing peptides that show an increase in abundance-corrected Cys reactivity (150) are derived from known Zn- and Fe-S-cluster proteins, revealing a significant decrease in metal occupancy in the proteome. Myriad cell processes are compromised by undermetalation of the metalloproteome, including enzymes that function in the TCA cycle and respiration, GTP metabolism, ribosome remodeling, tRNA charging, and proteostasis. A direct comparison of a strain lacking the candidate metallochaperone ZigA (∆zigA) with the wild-type strain reveals that the loss of ZigA is effectively silent in this assay. We conclude that CP induces a widespread, negative impact on the metalation status of the metalloproteome that results in a significant nutrient limitation response.
HostingRepository	PRIDE
AnnounceDate	2026-04-11
AnnouncementXML	Submission_2026-04-11_10:42:42.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Bak
SpeciesList	scientific name: Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377); NCBI TaxID: NEWT:400667;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-29 11:40:11	ID requested
⏵ 1	2026-04-11 10:42:42	announced

10.1021/ACSCHEMBIO.5C00753;

submitter keyword: Iron-sulfur cluster, ZigA, Acinetobacter baumannii, Chemoproteomics, Calprotectin,Zinc

Eranthie Weerapana
contact affiliation	Boston College, Chemistry
contact email	eranthie@bc.edu
lab head
Daniel Bak
contact affiliation	Boston College
contact email	bakd@bc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067881
     1. Label: PRIDE project
     2. Name: Metalloproteome remodeling in calprotectin-stressed Acinetobacter baumannii using chemoproteomics