PXD067878 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Novel asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus |
| Description | How essential asymmetries are built into icosahedral virus to facilitate directional genome egress is unknown. Here we capture the structure and dynamics of disassembly intermediates of an icosahedral virus. We reveal localized RNA density poised for release beneath 5-fold axes on one half of the viral particle. The asymmetric distribution of RNA is associated with a unique interchain isopeptide bond (Glu-Lys) that flanks RNA interacting loci on the capsid protein N-terminus. This singular isopeptide bond confers an essential 'loaded-die' feature that steers directional genome egress into target host cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-06 |
| AnnouncementXML | Submission_2025-11-06_13:06:51.996.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sean Braet |
| SpeciesList | scientific name: Turnip crinkle virus; NCBI TaxID: NEWT:11988; |
| ModificationList | isopeptide crosslinked residues; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse; Synapt MS |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-29 07:50:08 | ID requested | |
| ⏵ 1 | 2025-11-06 13:06:52 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Turnip Crinkle Virus,HDXMS, RNA Egress, Asymmetry, Isopeptide Bond |
Contact List
| Ganesh S. Anand |
|---|
| contact affiliation | Pennsylvania State University |
| contact email | gsa5089@psu.edu |
| lab head | |
| Sean Braet |
|---|
| contact affiliation | Penn State |
| contact email | smb6745@psu.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD067878 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067878
- Label: PRIDE project
- Name: Novel asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus
