PXD067840

PXD067840 is an original dataset announced via ProteomeXchange.

Title	Standardized Sample Preparation of Paediatric Bronchoalveolar Lavage Fluid for Mass Spectrometry-based Proteomic Analysis
Description	This study establishes a standardized and reproducible workflow for LC-MS/MS-based proteomic analysis of paediatric bronchoalveolar lavage fluid (BALF). Using minimal sample volumes, we systematically compared preparation strategies and identified a simplified protocol that maximizes protein recovery and coverage, supporting future large-scale biomarker and mechanistic studies in paediatric respiratory diseases.
HostingRepository	PRIDE
AnnounceDate	2025-11-21
AnnouncementXML	Submission_2025-11-21_05:47:00.335.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD067840
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	L-methionine removal; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 07:46:11	ID requested
⏵ 1	2025-11-21 05:47:01	announced

10.1186/s40348-025-00205-0;

Freitag N, Schramm D, Stefanski A, Schroeter CB, St, ü, hler K, Poschmann G, Driessen MD, Standardized sample preparation of paediatric bronchoalveolar lavage fluid for mass spectrometry based proteomic analysis. Mol Cell Pediatr, 12(1):21(2025) [pubmed]

10.6019/PXD067840;

submitter keyword: Paediatric bronchoalveolar lavage fluid

BALF

proteomics

LC-MS/MS

sample preparation

Orbitrap Fusion Lumos

biomarker discovery

respiratory disease

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine Heinrich Heine University Düsseldorf Universitätsstraße 1 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD067840

PRIDE project URI

• PRIDE
  1. PXD067840
     1. Label: PRIDE project
     2. Name: Standardized Sample Preparation of Paediatric Bronchoalveolar Lavage Fluid for Mass Spectrometry-based Proteomic Analysis