PXD067839

PXD067839 is an original dataset announced via ProteomeXchange.

Title	3D minichannels morphed tough grafts for repairing segmental tracheal defects
Description	Although tissue engineering studies have proven the feasibility of “chondrocyte-seeded scaffolds” for the formation of cartilaginous tracheal grafts in vivo, the use of these scaffolds is highly limited in humans owing to the scarcity of young chondrocytes and the dedifferentiation of in vitro expanded chondrocytes. In this study, we proposed a clinically operable approach to fabricate vascularized tracheal grafts through minced cartilage tissues and allogenous adipose derived mesenchymal stem cells and examined their behavior in tracheal defects in rabbit and porcine models. Using a template-sacrificing approach, we fabricated 3D-shaped (PCL-PGS) minichannels to enable the injection of "adipose stem cells - cartilage granules - platelet-rich plasma" constructs, while allowing nutritional perfusion from the wrapped fascia. Two weeks after ectopic transplantation, the grafts became vascularized and exhibited mechanical strength comparable to that of the native trachea. These grafts were subsequently anastomosed end-to-end to tracheal defects in both the rabbit and porcine models. Histological analysis revealed that the 3D-shaped "CG-ADSCs-PRP" compounds reassembled into cartilage rings and integrated with vascularized fascia, supporting animal survival for up to 67 days. ADSCs significantly enhanced the survival of cartilage granules within the morphed channels, primarily through increased glycolysis in chondrocytes.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-31_05:18:59.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinchi Zhang
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: NEWT:9986;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 07:41:05	ID requested
⏵ 1	2026-08-31 05:19:00	announced

Dataset with its publication pending

submitter keyword: Tracheal reconstruction

3D bioprinting

Adipose-derived stem cells

Xinchi Zhang
contact affiliation	State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Oral & Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi’ an, PR China.
contact email	zhangxc980812@163.com
lab head
Xinchi Zhang
contact affiliation	School of Stomatology, The Fourth Military Medical University
contact email	zhangxc980812@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD067839

PRIDE project URI

• PRIDE
  1. PXD067839
     1. Label: PRIDE project
     2. Name: 3D minichannels morphed tough grafts for repairing segmental tracheal defects