PXD067838

PXD067838 is an original dataset announced via ProteomeXchange.

Title	Adaptive Responses of Genome-Reduced Bacillus subtilis During Enzyme Secretion in a Breathing Vessel Culture
Description	The utilisation of microbes for the production of enzymes and pharmaceutical proteins is an important step towards a sustainable future. However, industrial fermentation processes require energy for agitation, aeration and cooling. This energy requirement increases with the increasing microbial cell mass. To address this technological challenge, we previously developed a so-called ‘breathing’ fermentation vessel that allows effective gas exchange. The present study aimed to explore the potential application of the breathing vessel for enzyme production by the Gram-positive bacterial cell factory Bacillus subtilis. In particular, we compared production of the secreted α-amylase AmyQ by the genome-reduced B. subtilis strain IIG-Bs-27-31 during parallel culturing in breathing vessels and shake flasks. Enzyme yields and the cellular and extracellular proteome compositions were compared.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:14:30.046.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: NEWT:224308;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 07:31:21	ID requested
⏵ 1	2026-02-22 17:14:31	announced

Ö, ktem A, Yokoyama K, Wu Y, Grilli F, Maa, ß S, Ishikawa S, Yotsuya M, Sato R, van Dijl JM, Yoshida KI, Adaptive responses of genome-reduced Bacillus subtilis during enzyme secretion in a breathing vessel culture. Microb Cell Fact, 25(1):(2026) [pubmed]

10.1186/s12934-026-02942-x;

submitter keyword: proteomics,Bacillus subtilis, industrial enzyme production, stress response, ‘breathing’ fermenter, genome reduction

Dörte Becher
contact affiliation	Department of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD067838

PRIDE project URI

• PRIDE
  1. PXD067838
     1. Label: PRIDE project
     2. Name: Adaptive Responses of Genome-Reduced Bacillus subtilis During Enzyme Secretion in a Breathing Vessel Culture