PXD067833

PXD067833 is an original dataset announced via ProteomeXchange.

Title	Insights into the biodegradation of two persistent fluorinated fungicides by coupling metabolic modelling with metaproteogenomics
Description	Epoxiconazole (EPO) and fludioxonil (FLU) are fluorinated fungicides characterized by their extremely high environmental persistence and ecotoxicity. Given their decades-old use in the agrochemical sector, EPO and FLU have become frequent pollutants of terrestrial and aquatic ecosystems. And yet, not much is known regarding how these pesticides biodegrade in the natural environment and/or how to develop suitable bioremediation approaches capable of tackling their inherent recalcitrance. As a result, this work focused on providing new insights into the bacterial degradation of EPO and FLU, by surveying the catabolic activity of a previously obtained EPO-enriched bacterial consortium through chemical and metaproteogenomic analyses guided by different metabolic modelling tools. The bacterial consortium was capable of extensively degrading EPO and FLU in 21 days, with fungicide removals of over 90 % and defluorination efficiencies of 55 to 80 %, but none of the subproducts predicted for either pesticide were identified by ESI-LC-MS/MS. Despite this, the combination of metabolic modelling tools and metaproteogenomic surveys suggested that EPO and FLU were first attacked in their N-heterocyclic moieties and that the targets of defluorination were the resulting aromatic fluorinated intermediates. This catabolic cascade is consistent with the experimental data gathered in this study and with the existing literature on this topic. Also, the degrading consortium remained surprisingly stable at the taxonomical and functional levels, highlighting its catabolic plasticity in biodegrading and defluorinating two chemically distinct fluorinated compounds. This work offers a conceptual framework with novel observations that can guide future efforts to further elucidate the pathways of microbial transformation of these pesticides, ultimately contributing for better environmental risk management practices for these pollutants.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_03:13:59.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diogo Alexandrino
SpeciesList	scientific name: metagenomes; NCBI TaxID: NEWT:408169;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 05:54:36	ID requested
⏵ 1	2026-01-23 03:14:00	announced

10.1038/s41598-025-31941-y;

Alexandrino DAM, Semedo M, Cao W, Azevedo J, Magalh, ã, es C, Os, ó, rio H, Jia Z, Campos A, Mucha AP, Almeida CMR, Carvalho MF, Insights into the biodegradation of two persistent fluorinated fungicides by coupling metabolic modelling with metaproteogenomics. Sci Rep, 16(1):2126(2026) [pubmed]

submitter keyword: Epoxiconazole

Fludioxonil

Metagenomics

Metaproteomics

Defluorination

Diogo Alexandrino
contact affiliation	Microbial Biodegradation and Bioprospection Research Group, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos s/n, 4450-208, Matosinhos, Portugal
contact email	dalexandrino@ciimar.up.pt
lab head
Diogo Alexandrino
contact affiliation	CIIMAR - University of Porto; Department of Environmental Health, P.Porto
contact email	dalexandrino@ciimar.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067833

PRIDE project URI

• PRIDE
  1. PXD067833
     1. Label: PRIDE project
     2. Name: Insights into the biodegradation of two persistent fluorinated fungicides by coupling metabolic modelling with metaproteogenomics