⮝ Full datasets listing
PXD067830
PXD067830 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | GPCR kinases shape ACKR4 functions via differential C-terminal phosphorylation |
| Description | Atypical chemokine receptor 4 (ACKR40+) is a scavenger receptor that regulates the availability of chemokines, including CCL19, and consequently the responsiveness of their classical G protein coupled receptors. In contrast to classical chemokine receptors, ACKR4 is completely biased towards beta-arrestins and does not couple to G proteins. Here, we show that ACKR4 in its apo state constitutively pre-associates with beta-arrestins and cycles between the plasma membrane and endosomal compartments. We identify distinct serine and threonine residues in the tail region of ACKR4 involved in regulating steady-state receptor trafficking and chemokine uptake, and that a C-terminal serine/threonine cluster is key for both ligand-mediated beta-arrestin recruitment and efficient chemokine uptake. Moreover, different serine/threonine clusters in the tail region of ACKR4 account for steady-state and chemokine-driven association of the four non-visual GPCR kinases (GRKs), which differentially phosphorylate two serine and one threonine residues. We show that GRK5/6 primarily phosphorylate ACKR4 in the absence of chemokines, and that CCL19 stimulation recruits GRK2/3 to enhance ACKR4 phosphorylation. Notably, we found that GRK2 and GRK3 can interact with the heterotrimeric G protein without the need of its activation. Finally, we show that the C-terminal serine/threonine cluster of ACKR4 and GRK2 play leading roles in beta-arrestin recruitment and CCL19 internalisation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-13 |
| AnnouncementXML | Submission_2026-04-13_07:11:47.111.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Roland Bruderer |
| SpeciesList | scientific name: human; |
| ModificationList | Phospho |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-28 05:10:33 | ID requested | |
| ⏵ 1 | 2026-04-13 07:11:47 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: gpcr, DatasetType:Proteomics |
Contact List
| David Legler | |
|---|---|
| contact affiliation | Institute of Cell Biology and Immunology Thurgau |
| contact email | daniel.legler@bitg.ch |
| lab head | |
| Roland Bruderer | |
| contact affiliation | Biognosys AG |
| contact email | roland.bruderer@biognosys.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098965/ |
