PXD067808

PXD067808 is an original dataset announced via ProteomeXchange.

Title	Pseudo-acetylation of ACTC1 K326 and K328 promotes dysinhibition of reconstituted human cardiac thin filaments
Description	Tropomyosin (Tpm) regulates cardiac thin filament function by restricting myosin access through electrostatic interactions with actin - particularly involving residues K326 and K328 on each actin monomer. Acetylation at these lysines neutralizes charge, destabilizing Tpm’s inhibitory position and favoring myosin engagement. We hypothesize that mimicking this modification via a K to Q mutation would enhance contractility due to Tpm’s disinhibition. To determine whether cardiac actin amino acids K326 and 328 are acetylated in human cardiac tissue, we performed in-gel tryptic digestion of the major 42 kDa SDS-PAGE band (actin) from non-failing, donor cardiac homogenate replicates, and subsequently subjected the peptides to LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-30_16:11:36.201.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD067808
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sogol Sedighi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-27 15:01:40	ID requested
⏵ 1	2026-08-30 16:11:37	announced

Chitre K, Karpicheva OE, King CJ, Rynkiewicz MJ, Fenwick AJ, Dawson JF, Foster DB, Lehman W, Cammarato A, Pseudo-acetylation of ACTC1 K326 and K328 promotes dysinhibition of reconstituted human cardiac thin filaments. J Mol Cell Cardiol, 212():10-15(2026) [pubmed]

10.6019/PXD067808;

10.1016/j.yjmcc.2025.12.008;

submitter keyword: Actin, Human, Acetylation

Anthony Cammarato
contact affiliation	Division of Cardiology Department of Medicine Johns Hopkins University Baltimore, MD 21205
contact email	acammar3@jhmi.edu
lab head
Sogol Sedighi
contact affiliation	Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
contact email	ssedigh2@jh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067808
     1. Label: PRIDE project
     2. Name: Pseudo-acetylation of ACTC1 K326 and K328 promotes dysinhibition of reconstituted human cardiac thin filaments