PXD067807

PXD067807 is an original dataset announced via ProteomeXchange.

Title	Profiling Neuropeptides in Adrenomedullary Chromaffin Cells: Insights into Regulated Release via Electrical and Chemical Stimulation
Description	Despite longstanding recognition that the adrenal medulla is a major source of catecholamines and opioid peptides, the dynamic co-release of these signaling molecules—and the full complexity of their molecular forms—remains poorly understood. This study addresses this gap by integrating real-time electrochemical detection with tandem mass spectrometry to map and quantify the neurochemical complexity of opioid peptide signaling from adrenal chromaffin cells. Using FSCV, we provide evidence for stimulus-evoked co-release of met-enkephalin and catecholamines from single chromaffin cells (and adrenal slices?). With MS, we show that proenkephalin-derived peptides dominate the adrenal peptidome and that extended enkephalin peptides—such as BAM-18 and BAM-22—are not only present in cell content but are actively released.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:31:59.829.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD067807
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elena Romanova
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	amidated residue; 2-pyrrolidone-5-carboxylic acid (Glu); phosphorylated residue; monohydroxylated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-27 14:19:51	ID requested
⏵ 1	2026-06-28 16:32:00	announced

10.1021/acschemneuro.6c00180;

Berger JM, Denison JD, De Alwis AC, Sumner HP, McCarty GS, Romanova EV, Sweedler JV, Sombers LA, Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells. ACS Chem Neurosci, 17(10):1989-2003(2026) [pubmed]

10.6019/PXD067807;

submitter keyword: Cyclic voltammetry, Proenkephalin, trapped ion mobility spectrometry, neuropeptides release, bovine adrenal slices

Jonathan V. Sweedler
contact affiliation	Department of Chemistry University of Illinois 71 RAL, Box 63-5 600 South Mathews Avenue Urbana, IL 61801, USA
contact email	jsweedle@illinois.edu
lab head
Elena Romanova
contact affiliation	University of Illinois
contact email	romanova@illinois.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067807

PRIDE project URI

• PRIDE
  1. PXD067807
     1. Label: PRIDE project
     2. Name: Profiling Neuropeptides in Adrenomedullary Chromaffin Cells: Insights into Regulated Release via Electrical and Chemical Stimulation