PXD067801

PXD067801 is an original dataset announced via ProteomeXchange.

Title	Novel peptides from the edible bivalve Ruditapes decussatus target apoptosis, autophagy, and FGF19-FGFR4 signaling in human cancer cell lines
Description	Bioactive peptides have recently emerged as promising candidates for cancer treatment due to their selective cytotoxicity toward cancer cells. The bivalve mollusk Ruditapes decussatus contains bioactive compounds that have not been thoroughly investigated for their potential anticancer properties. In this study, isolation and purification of peptide mixtures from R. decussatus was performed using FPLC chromatography followed by de novo sequence analysis. Using de novo peptide sequencing, a total of 135 peptides (ranging from 2,681.6 to 5,925.12 Da) were identified, of which 57 peptides (42%) were predicted to exhibit anticancer potential upon analysis with AntiCP 2.0, highlighting their possible therapeutic utility. Additionally, fractions were tested against liver (HepG2) and colorectal (HT-29) cancer cell lines, as well as normal human hepatocytes and VERO (obtained from kidney) cells, to evaluate their cytotoxic effects. Fractions 2 and 3 showed significant anticancer activity against both cancer cell lines, while exhibiting minimal cytotoxicity toward normal cells. These fractions induced apoptosis, as evidenced by the downregulation of Bcl-2 and upregulation of caspase-3, and also activated autophagy, marked by increased Beclin-1 expression. Flow cytometry analysis revealed enhanced apoptotic cell death and G1/S phase cell cycle arrest in the treated cancer cells. Morphological analysis further confirmed the presence of apoptotic changes. Overall, the peptides derived from R. decussatus demonstrated the ability to induce apoptosis and cell cycle arrest in cancer cells, with a highly selective effect on colorectal carcinoma, suggesting their potential as anticancer agents for further investigation.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:05:32.716.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ahmed Hussein
SpeciesList	scientific name: Ruditapes decussatus; NCBI TaxID: NEWT:104385;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-27 09:33:28	ID requested
⏵ 1	2025-10-05 16:05:33	announced

10.1038/s41598-025-20094-7;

Hussein AAA, Salem MB, ElZallat M, Ghoname SI, Habib MR, Hammam OA, El-Dabaa E, Okasha H, Novel peptides from the edible bivalve Ruditapes decussatus target apoptosis, autophagy, and FGF19-FGFR4 signaling in human cancer cell lines. Sci Rep, 15(1):34283(2025) [pubmed]

submitter keyword: Bioactive peptides, Natural agents, Anticancer activity, Mass spectrometry,Bivalves

Ahmed Hussein
contact affiliation	Medical Malacology, Theodore Bilharz Research Institute, Giza, Egypt
contact email	ahmed.abdelazeez@science.suez.edu.eg
lab head
Ahmed Hussein
contact affiliation	Mdical Malacology Department, Theodor Bilharz Research Institute (TBRI)
contact email	ahmed.abdelazeez@science.suez.edu.eg
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067801
     1. Label: PRIDE project
     2. Name: Novel peptides from the edible bivalve Ruditapes decussatus target apoptosis, autophagy, and FGF19-FGFR4 signaling in human cancer cell lines