PXD067788

PXD067788 is an original dataset announced via ProteomeXchange.

Title	Identify proteins that interact with hsa_circ_0001910 using mass spectrometry.
Description	Circular RNAs (circRNAs) have emerged as critical regulators in various biological processes through their interactions with RNA-binding proteins. To investigate the protein interactions of hsa_circ_0001910, a circRNA of potential functional significance, we designed a specific RNA pulldown probe targeting this molecule. Using this probe, we performed RNA pull-down assays to enrich proteins that directly or indirectly bind to hsa_circ_0001910. The pulled-down protein complexes were subsequently separated and detected by Western blotting to confirm the presence of specific protein candidates. For comprehensive and unbiased protein identification, the enriched protein samples were further subjected to mass spectrometry (MS)-based proteomic analysis. This approach allows for the systematic identification and quantification of proteins that interact with hsa_circ_0001910, providing insights into its molecular functions and potential roles in cellular pathways.
HostingRepository	PRIDE
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-02_16:21:24.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fei Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	co-eluting ion

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-27 02:27:53	ID requested
⏵ 1	2025-11-02 16:21:25	announced

10.3389/fimmu.2025.1665608;

Wu M, Du Y, Dong Y, Zheng Y, Gu L, Tang X, Yan L, Ji H, Sang Y, Liu F, circWWC3 enhances the progression of triple-negative breast cancer by interacting with vimentin to regulate the secretion of CSF2. Front Immunol, 16():1665608(2025) [pubmed]

submitter keyword: Breast cancer， hsa_circ_0001910，Proteomics profiling

Fei Liu
contact affiliation	The Fourth Hospital of Hebei Medical University
contact email	kyzxlf@hotmail.com
lab head
Fei Liu
contact affiliation	the fourth hospital of hebei medical university
contact email	kyzxlf@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD067788

PRIDE project URI

• PRIDE
  1. PXD067788
     1. Label: PRIDE project
     2. Name: Identify proteins that interact with hsa_circ_0001910 using mass spectrometry.