PXD067762

PXD067762 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics of Bark and Xylem Provides Insights into Age-Dependent Corticular Photosynthesis in Eucalyptus grandis
Description	Eucalyptus species are vital for the global forestry industry, known for their rapid growth, adaptability, and high biomass production. Beyond their economic importance, eucalyptus plantations contribute to carbon sequestration, playing a crucial role in climate change mitigation by capturing atmospheric CO₂ and storing it as biomass. Vascular tissues, such as phloem and xylem, represent the primary bioproducts of interest in woody plants, exhibiting unique metabolic adaptations to meet the energetic demands for growth and maintenance. However, understanding the metabolic mechanisms that sustain growth, particularly under hypoxic conditions within woody tissues, remains unknown. Insights into the differential regulation of proteins across vascular tissues at contrasting developmental stages can facilitate the monitoring of metabolic responses during xylogenesis and support plant breeding programs. This study investigates the role of corticular photosynthesis in sustaining energy metabolism within eucalyptus stems by analyzing bark and xylem tissues from 4- and 12-year-old Eucalyptus grandis clonal plants. To achieve this, we employed fluorescence microscopy to quantify chloroplast abundance across bark developmental stages and performed proteomic profiling of both tissues. The results revealed a higher number of chloroplasts in younger bark, contrasting with their absence in xylem. A total of 3,113 non-redundant proteins were identified, and enrichment analysis demonstrated a consistent hypoxic response across tissues and ages, alongside age-specific metabolic processes. Proteomic analysis further uncovered distinct proteoforms abundance patterns associated with glycolysis, the tricarboxylic acid cycle, and fermentation pathways. Notably, we observed the presence of differential proteoforms abundance of alcohol dehydrogenase and aldehyde dehydrogenase in both xylem tissues and younger bark, indicating a metabolic shift toward fermentative pathways under hypoxic conditions. Additionally, younger bark tissues exhibited higher abundance of proteins linked to the Calvin-Benson cycle, suggesting active corticular photosynthesis that enhances oxygen availability for mitochondrial respiration in the cambial zone. These findings underscore the adaptive metabolic strategies of eucalyptus stems, emphasizing the pivotal role of corticular photosynthesis in supporting energy production, growth, and wood formation.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:43:55.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felipe Silva
SpeciesList	scientific name: Eucalyptus grandis; NCBI TaxID: NEWT:71139;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-26 14:29:17	ID requested
⏵ 1	2026-02-22 17:43:56	announced

10.3389/fpls.2026.1701400;

da Silva FAR, de Lima DC, Labate MTV, Budzinski IGF, Cataldi TR, Labate CA, . Front Plant Sci, 17():1701400(2026) [pubmed]

submitter keyword: carbon metabolism, proteomics, corticular photosynthesis,eucalyptus, fermentation, glycolysis

Carlos Alberto Labate
contact affiliation	Max Feffer Laboratory of Plant Genetics, Department of Genetics, College of Agriculture “Luiz de Queiroz”, University of São Paulo, Av. Pádua Dias 11, CEP 13418-900, Piracicaba-SP, Brazil
contact email	calabate@usp.br
lab head
Felipe Silva
contact affiliation	University of São Paulo
contact email	far.silva@unesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067762
     1. Label: PRIDE project
     2. Name: Comparative Proteomics of Bark and Xylem Provides Insights into Age-Dependent Corticular Photosynthesis in Eucalyptus grandis