PXD067746

PXD067746 is an original dataset announced via ProteomeXchange.

Title	Variability in Presence, Abundance, and Timing of Bovine Milk- and Soy Beverage-Derived Peptides in Human Milk After Maternal Bovine Milk and Soy Beverage Consumption: A Randomized, Cross-over, Dietary Intervention Trial
Description	BACKGROUND: Food-borne allergens in human milk (HM) may cause allergic responses in HM-fed infants, but variability of allergen transfer complicates recommendations for individuals nursing food-allergic infants. OBJECTIVE: We aimed to identify bovine- and soy-derived peptides in HM after maternal elimination and reintroduction of bovine milk (BM) and soy beverage (SB). METHODS: In this randomized, cross-over, dietary intervention trial, 38 lactating participants underwent 2 study phases, each including a 5-day diet elimination, 3-day diet intervention, and 2-day washout. Each diet intervention required daily consumption of increasing amounts of BM or SB (175, 295, and 415 mL). Peptidomics analysis was performed on a subset of HM samples (24 participants) collected after dietary elimination, and 2 and 4 h after BM/SB consumption (415 mL). Peptides were isolated via ethanol precipitation and C18 solid-phase extraction, analyzed by LC-MS/MS, and identified with Proteome Discoverer. RESULTS: We identified 121 bovine-derived peptides (associated with 6 proteins) in HM collected during the BM phase. From most to least abundant, these proteins were β-lactoglobulin, κ-casein, αs1-casein, β-casein, α-lactalbumin protein variant D, and glycosylation-dependent cell adhesion molecule 1. Generalized linear mixed models demonstrated differences in relative abundance for 14 peptides when comparing before, and 2 and 4 h after BM consumption. We identified 8 peptides of possible soy origin in HM collected during the SB phase, but they were not matched to parent proteins with adequate confidence. CONCLUSIONS: The relative abundance of some BM-derived peptides, while low overall, may differ in human milk collected after maternal BM dietary elimination compared to 2 and 4 h after BM consumption. Five days of dietary elimination may not be adequate for the elimination of BM-derived peptides or low levels of these non-human peptides may be present in HM from other sources. Soy-derived peptides
HostingRepository	PRIDE
AnnounceDate	2025-10-21
AnnouncementXML	Submission_2025-10-21_13:48:58.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janet Williams
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; scientific name: Glycine max; NCBI TaxID: NEWT:3847;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-26 08:01:10	ID requested
⏵ 1	2025-10-21 13:48:59	announced

10.3389/FNUT.2025.1642177;

submitter keyword: peptidomics,nutrition, allergen, breastfeeding, infant, diet, lactation, maternal

Michelle McGuire
contact affiliation	University of Idaho
contact email	smcguire@uidaho.edu
lab head
Janet Williams
contact affiliation	University of Idaho
contact email	janetw@uidaho.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067746
     1. Label: PRIDE project
     2. Name: Variability in Presence, Abundance, and Timing of Bovine Milk- and Soy Beverage-Derived Peptides in Human Milk After Maternal Bovine Milk and Soy Beverage Consumption: A Randomized, Cross-over, Dietary Intervention Trial