⮝ Full datasets listing
PXD067744
PXD067744 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of complex core-fucosylated glycans on proteins associated with metastatic progression in breast cancer |
| Description | Evidence suggests that cancer cells, including breast cancer, have perturbed metabolisms and modified the use of sugars leading to unique expression patterns of N-glycans. In particular, core-fucosylation, a fucose residue added to the core N-glycan structural sugar ,is a major N-glycan modification that is added to proteins and it is significantly upregulated in breast cancer. Analysis of human tumor microarray (TMA) samples by imaging mass spectrometry (iMS) identified core-fucosylated N-glycans (ie. the product of having high FUT8) that are strongly associated with breast cancer metastasis8. Inhibition of core-fucosylation in metastatic breast cancer cells inhibits cell migration, invasion, and metastatic signaling. ere, we report methods to use the the lectin rAALN224Q, which was previously characterized to bind specifically to complex core-fucosylated N-glycans, and show that we can successfully use rAALN224Q (heretofore referred to as N244Q) to enrich proteins and identify glycopeptides using high resolution LC-MS/MS . Initial N244Q enrichment and glycoproteomic analysis using 4T1 cells in the presence or absence of 2FF resulted in identification of core-fucosylated proteins with significant relationship to metastasis related pathways. Further analysis identifies complex core-fucosylated N-glycans on specific proteins including LRP1, THBS1, and cMet. Blockade of fucosylation alters the interactome of these core-fucosylated proteins leading to changes in their signaling capacity. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-29_08:28:27.876.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | unknown modification |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-26 06:44:05 | ID requested | |
| ⏵ 1 | 2026-06-29 08:28:28 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: core-fucosylation, metastatic, breast cancer, complex core-fucosylated N-glycans, glycopeptide LC-MS/MS, DatasetType:Proteomics |
Contact List
| Emma Doud | |
|---|---|
| contact affiliation | Indiana University School of Medicine |
| contact email | edoud@iu.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098938/ |
