PXD067739 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BFP pulldown of BFP-NUDT5 HAP1 MTHFD1-K56R cells |
| Description | We performed BFP pulldowns with BFP-tagged NUDT5 in HAP1 harboring the MTHFD1 K56R mutation to find interactors of NUDT5. We performed the study under various medium conditions, including full IMDM medium (FULL), IMDM with dialysed FBS (DIA) and DIA supplemented with 50µM adenosine to investigate potential changes of interactors upon different medium conditions. Additionally, we performed Pulldowns with a catalytic inactive NUDT5 variant (E112Q), to check whether its catalytic activity has an influence in the interactome. As a control, we used HAP1 MTHFD1 K56R NUDT5 KO cells. Experiments were performed in duplicates |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-07 |
| AnnouncementXML | Submission_2025-11-07_06:35:38.505.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD067739 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Nara Marella |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-26 05:11:45 | ID requested | |
| ⏵ 1 | 2025-11-07 06:35:39 | announced | |
Publication List
Keyword List
Contact List
| Stefan Kubicek |
|---|
| contact affiliation | Head, Molecular Discovery Platform CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Lazarettgasse 14, AKH BT 25.3 1090 Vienna, Austria www.cemm.at |
| contact email | skubicek@cemm.at |
| lab head | |
| Nara Marella |
|---|
| contact affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences |
| contact email | nara.marella@outlook.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD067739 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067739
- Label: PRIDE project
- Name: BFP pulldown of BFP-NUDT5 HAP1 MTHFD1-K56R cells
