PXD067737 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Targeted quantitative analysis of specific proteins in cytosolic, mitochondrial, and nuclear fractions using PRM |
| Description | We present a targeted proteomic approach using parallel reaction monitoring (PRM) to assess the enrichment of cytosolic, mitochondrial, and nuclear fractions obtained from liver samples using commercial isolation kits. For mitochondrial fraction, CPS1 and ATP5F1 were chosen. For the cytosolic fraction, HSP90AB1 and AHCY were selected. For the nuclear fraction, LMNA and NPM1 were used as markers. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-13 |
| AnnouncementXML | Submission_2026-04-12_16:26:37.522.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alejandra Delgado Sequera |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-08-26 04:31:37 | ID requested | |
| ⏵ 1 | 2026-04-12 16:26:38 | announced | |
Publication List
| 10.1021/acs.jproteome.5c00697; |
| Delgado-Sequera A, Paradela A, Corrales FJ, Targeted Quantitative Analysis of Specific Proteins in Cytosolic, Mitochondrial, and Nuclear Fractions Using PRM. J Proteome Res, 25(4):2183-2192(2026) [pubmed] |
Keyword List
| submitter keyword: mitochondrial enrichment, liver,PRM, subcellular fractionation, target proteomic |
Contact List
| Fernando Corrales |
|---|
| contact affiliation | CNB-CSIC |
| contact email | fcorrales@cnb.csic.es |
| lab head | |
| Alejandra Delgado Sequera |
|---|
| contact affiliation | CNB-CSIC |
| contact email | alejandra.delgado@gm.uca.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD067737 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD067737
- Label: PRIDE project
- Name: Targeted quantitative analysis of specific proteins in cytosolic, mitochondrial, and nuclear fractions using PRM
