PXD067725

PXD067725 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Phosphoproteomic Profiling Reveals Sex-Specific Regulatory Mechanisms in HrasG12V-Driven Hepatocarcinogenesis
Description	To identify common and sex-specific phosphorylation dynamics during hepatocarcinogenesis in Hras12V transgenic mice (Ras-Tg). We constructed a phosphoproteomic database using male/female tumor (MT/FT), precancerous (MP/FP), and wild-type liver tissues (MW/FW) from Ras-Tg, validated via parallel reaction monitoring (PRM). Comparative analysis and hierarchical clustering were employed to delineate shared and sex-stratified phosphorylation signatures in hepatocellular carcinoma (HCC) development. PRM-validated phosphoproteomic profiling quantified 5,410 phosphorylation sites across 2,427 proteins. Analysis of common features revealed nuclear-enriched phosphoprotein accumulation in tumors versus precancerous/wild-type tissues (T vs P/W). KEGG pathway analysis identified consistently dysregulated pathways including MAPK signaling, focal adhesion, and protein digestion/absorption. Protein-protein interaction (PPI) network analysis of shared phosphoproteins pinpointed key regulators (Alb, Hspa5, Psn). Sex-specific analyses demonstrated distinct phosphorylation patterns: males exhibited extensive membrane protein phosphorylation alterations, while females showed predominant cytoplasmic modifications. KEGG pathway mapping revealed male-biased dysregulation in Ras signaling, mTOR pathways, and actin cytoskeleton regulation. Functional annotation indicated greater complexity of phosphorylated proteins in males. Notably, kinase/phosphatase activity-related phosphorylation events were more prevalent in males, suggesting enhanced phosphorylation-mediated signaling dynamics. This study establishes the first sexual dimorphism-aware phosphoproteomic resource for Ras-driven hepatocarcinogenesis, systematically characterizing conserved and sex-divergent phosphorylation networks. The findings provide mechanistic insights into gender disparities in HCC progression and potential therapeutic targeting.
HostingRepository	iProX
AnnounceDate	2025-08-26
AnnouncementXML	Submission_2025-08-25_23:41:54.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiling Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-25 23:41:28	ID requested
⏵ 1	2025-08-25 23:41:55	announced

Dataset with its publication pending

submitter keyword: myasthenia gravis, proteomics, biomarkers, complement coagulation cascade, Cytoskeleton

Huiling Li
contact affiliation	Dalian Medical University
contact email	lhl@dmu.edu.cn
lab head
Huiling Li
contact affiliation	Dalian Medical University
contact email	754037170@qq.com
dataset submitter

iProX dataset URI