PXD067716

PXD067716 is an original dataset announced via ProteomeXchange.

Title	A Phosphoinositide Interacting Protein Coordinates Stress Precursor Activities
Description	Samd14 coordinates stem cell factor/Kit receptor signaling and is crucial for the survivalof red blood cel precursors within anemia contexts. Here we present new findings that Samd14 expression is needed to maintain balanced autophagy in red blood cell precursors following acute anemia. Autophagy gene signatures and protein levels are markedly altered withinthe context of acute anemia when red blood cell precursors accelerate the process of erythropoiesis. Samd14 interacted via its SAM domain with phosphatidylinositol 3-phosphate (PI3P) which is an integral component of endosomal and autophagic membranes. We tested PI3Ps role in red blood cell differentiation using a small molecule inhibitor of the Class III PI 3-kinase VPS34, which is the sole kinase responsible for PI3P genesis. SAR405 treatment blocked red blood cell formation. In the absence of Samd14, higher doses of VPS34 inhibition were required to block erythroid differentiation. Given the critical roles of autophagy in normal differentiation, Samd14’s stress-dependent activation and roles in autophagy suggest that this mechanism is needed to maintain progenitor levels and balance the production of healthy, mature red blood cells.
HostingRepository	PRIDE
AnnounceDate	2026-02-11
AnnouncementXML	Submission_2026-02-11_09:00:39.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Anemia; NCBI TaxID: NCBITaxon:12939;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-25 18:51:09	ID requested
⏵ 1	2026-02-11 09:00:40	announced

Dataset with its publication pending

submitter keyword: autophagy,Samd14, red blood cells, phosphatidylinositol 3-phosphate

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska - Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067716
     1. Label: PRIDE project
     2. Name: A Phosphoinositide Interacting Protein Coordinates Stress Precursor Activities