PXD067713

PXD067713 is an original dataset announced via ProteomeXchange.

Title	5-hydroxymethylfurfural and isoverbascoside alleviate oxidative damage in insulinoma-1 and mouse insulinoma 6 cells by activating autophagy and inhibiting apoptosis
Description	In type 2 diabetes (T2DM), dysregulated glucose and lipid metabolism impairs cellular energy sensing and inhibits autophagy. This leads to accumulation of dysfunctional cellular components, promoting inflammation and oxidative stress, which ultimately activate the intrinsic apoptotic pathway and cell death. However, the regulatory mechanisms by which herbal and natural compounds modulate the balance between autophagy and apoptosis require further elucidation. This study explored the protective mechanisms of 5-Hydroxymethylfurfural (5-HMF) and isoverbascoside (10-640 μM, 24 hours) from RehmanniaeRadix Praeparata against H₂O₂-induced oxidative damage in insulinoma-1 (INS-1) and mouse insulinoma 6 (MIN6) β-cells through autophagy-apoptosis balance modulation, employing transcriptomics, proteomics, machine learning, and molecular biology approaches. Research demonstrated that compared to the H2O2 group, 5-HMF and isoverbascoside (P < 0.01) enhance β-cell survival rates, as evidenced by reduced apoptosis in flow cytometry (P < 0.01) and Hoechst 33342 staining assays, alongside increased LC3 protein fluorescence intensity. Transcriptomic and proteomic analyses revealed their modulation of autophagy and apoptosis-related differentially expressed genes, proteins and pathways. Western blot analysis showed that 5-HMF (P < 0.0001) and isoverbascoside (P < 0.0001) significantly increased Beclin-1 expression, compared with the H2O2 group. To explore autophagy's role, chloroquine (CQ), an autophagy inhibitor, was used with H2O2. 5-HMF (P < 0.0001, P < 0.0001 ) and isoverbascoside (P < 0.0001, P < 0.0001 ) significantly increased B-cell lymphoma 2 and decreased Bcl-2-associated X protein levels compared to the H2O2+CQ group. Those findings suggest that 5-HMF and isoverbascoside enhance the autophagic activity of pancreatic β cells, mitigate apoptosis induced by oxidative stress, improve cell survival and proliferation capabilities, and ultimately exert a protective effect in the context of T2DM.
HostingRepository	iProX
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-25_18:38:12.637.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuting Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-25 18:37:35	ID requested
⏵ 1	2025-08-25 18:38:13	announced

Dataset with its publication pending

submitter keyword: T2DM, Autophagy, Apoptosis, Pancreatic β cell, 5-Hydroxymethylfurfural, Isoverbascoside

Shuosheng Zhang
contact affiliation	College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine
contact email	zhangshuosheng@aliyun.com
lab head
Yuting Li
contact affiliation	College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine
contact email	2497147877@qq.com
dataset submitter

iProX dataset URI