PXD067709

PXD067709 is an original dataset announced via ProteomeXchange.

Title	Cysteine reactivity profiling identifies host regulators of Mycobacterium tuberculosis replication in human macrophages
Description	Innate immune cells such as monocytes and macrophages provide the earliest defense against infection by intracellular pathogens by initiating signaling pathways and restricting pathogen replication. However, the full complement of proteins that mediate cell-autonomous immunity remains incompletely defined. Here, we applied cysteine-directed activity-based protein profiling (ABPP) to map proteome-wide cysteine reactivity changes in THP-1 monocytes and primary human monocyte-derived macrophages during Mycobacterium tuberculosis (Mtb) infection. Across both cell types, we quantified 148 cysteine residues with altered reactivity. Genetic perturbation of a subset of proteins harboring these changes significantly impacted Mtb replication, revealing functional links between site-specific cysteine reactivity and antimicrobial defense. These data define previously unrecognized host protein changes during Mtb infection and provide a resource for investigating post-translational events that regulate innate immune responses to intracellular bacteria.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_18:16:36.839.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristen DeMeester
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-25 15:56:42	ID requested
⏵ 1	2026-03-01 18:16:37	announced

Neff JK, DeMeester KE, P, á, rraga Sol, ó, rzano PK, Suciu RM, Dix MM, Simon G, Gianakopoulos MA, Melillo B, Cravatt BF, Shiloh MU, Replication in Human Macrophages. ACS Infect Dis, 12(2):781-793(2026) [pubmed]

10.1021/acsinfecdis.5c00984;

submitter keyword: innate immunity, cysteine, macrophage,Mycobacterium tuberculosis, mass spectrometry, chemical proteomics, covalent probes

Benjamin Cravatt
contact affiliation	Scripps Research
contact email	cravatt@scripps.edu
lab head
Kristen DeMeester
contact affiliation	Lafayette College
contact email	demeestk@lafayette.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD067709

PRIDE project URI

• PRIDE
  1. PXD067709
     1. Label: PRIDE project
     2. Name: Cysteine reactivity profiling identifies host regulators of Mycobacterium tuberculosis replication in human macrophages