PXD067699

PXD067699 is an original dataset announced via ProteomeXchange.

Title	Deciphering Membrane Protein Complexes in Plasmodium falciparum Gametocytes via Cross-Linking Mass Spectrometry and Structural Modeling
Description	Malaria is a disease targeting the most vulnerable people. It is caused by the parasite Plasmodium falciparum, and is transmitted to humans by Anopheles mosquitos during a blood meal, who are in turn infected by the parasite if they feed on an infected human. While current eradication efforts focus on mosquito control, transmission blocking therapeutics will be critical for successful eradication. The parasite goes through several life stages and reproduces sexually. This reproduction is mediated by gametocytes, which are responsible for transmission from humans to mosquitos, and thus represent an excellent target for transmission blocking therapeutics. However, there is very little information on the composition and interactions of gametocyte-specific proteins, in particular membrane proteins. In order to fill this knowledge gap, we cultured the gametocytes and isolated their membrane fraction. We then used cross-linking mass-spectrometry as well as size-exclusion and strong-cation exchange fractionation to identify protein-protein interaction networks. The interactions identified encompass host-pathogen and pathogen-pathogen complexes, and are distributed among many cellular compartments. The results of this study extend the understanding of malaria gametocyte biology, and serve as a springboard to accelerate research into potentially transmission-blocking Malaria therapeutics.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_08:00:17.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Max Ruwolt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Plasmodium falciparum 58.1; NCBI TaxID: NEWT:1245012;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-25 07:13:33	ID requested
⏵ 1	2026-04-09 08:00:18	announced

Dataset with its publication pending

submitter keyword: gametocytes, malaria, cross-linking,plasmodium, alphafold

Jan Kosinski
contact affiliation	EMBL Hamburg Germany
contact email	jan.kosinski@embl.de
lab head
Max Ruwolt
contact affiliation	Leibniz-Forschungsinstitut for Molecular Pharmacology
contact email	ruwolt@fmp-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD067699

PRIDE project URI

• PRIDE
  1. PXD067699
     1. Label: PRIDE project
     2. Name: Deciphering Membrane Protein Complexes in Plasmodium falciparum Gametocytes via Cross-Linking Mass Spectrometry and Structural Modeling