PXD067673

PXD067673 is an original dataset announced via ProteomeXchange.

Title	In Situ Cross-linking Based Co-Fractionation Mass Spectrometry (XL-CoFrac-MS)
Description	We developed an in situ cross-linking coupled reversed-phase liquid chromatography-based co-fractionation mass spectrometry (XL-CoFrac-MS) platform to analyze drug-induced protein complex changes. A software tool, ChromaQuant, was built to automate chromatographic peak quantification. Using RS4;11 leukemia cells treated with the BCL-2 inhibitor ABT-199, we demonstrated the ability of the platform to detect BCL-2-associated protein complexes and characterize signaling pathway alterations. This approach enables efficient protein complex profiling and provides mechanistic insights into drug-induced apoptosis.
HostingRepository	iProX
AnnounceDate	2025-08-23
AnnouncementXML	Submission_2025-09-01_01:46:46.128.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-24 23:28:26	ID requested
⏵ 1	2025-09-01 01:46:46	announced

Liu W, Tang J, Yan G, Shen S, Gao M, Zhang X, A Chromatography-Guided Co-Fractionation Mass Spectrometry Strategy for Rapid Profiling of Drug-Perturbed Protein Complexes. Anal Chem, 97(35):19146-19156(2025) [pubmed]

submitter keyword: Cross-linking mass spectrometry, Co-fractionation, RPLC, Chromatographic profiling, Protein complexes, ABT-199, Apoptosis, BCL-2, Leukemia, ChromaQuant

Mingxia Gao
contact affiliation	Fudan University
contact email	mxgao@fudan.edu.cn
lab head
Wei Liu
contact affiliation	Fudan University
contact email	wliu21@m.fudan.edu.cn
dataset submitter

iProX dataset URI