PXD067665

PXD067665 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling of Oxidative Stress Response Proteins Using Methionine Sulfoxide-Inspired ABPP Probes
Description	Methionine oxidation is a significant signature of oxidative stress. In nature, oxidative stress from reactive oxygen species or reactive chlorine species (ROS/RCS) serves as a fundamental defense mechanism against pathogenic sources. However, previous tools for studying methionine oxidation have depended on sulfoxide reduction strategies, making them unsuitable for use under oxidative stress conditions. Moreover, no ABPP tools have been reported for studying the activity of cellular methionine sulfoxide reductases. In this paper, we present a cysteine-targeted chemical probe inspired by methionine sulfoxide for profiling oxidative stress response proteins, including methionine sulfoxide reductases. Our probe efficiently labels nucleophilic cysteines of methionine sulfoxide reductases and functions as an activity-based protein profiling tool. The probe demonstrated good reactivity and stability, making it applicable to both in vitro and in vivo experiments under oxidative stress conditions. We showed that our probe labeled oxidative stress-related proteins in E. coli under hypochlorite stress. Finally, we demonstrated that our ABPP probe offers complementary cysteine reactivity profiles compared to conventional probes, efficiently identifying proteins such as DJ-1 superfamily members that are poorly captured by iodoacetamide-alkyne. We believe that our probe can be used to reveal the biological mechanisms by which bacteria sense strong oxidants and endure oxidative stress, such as that encountered with neutrophils.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_17:54:07.482.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Seungmin Ahn
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-24 19:53:46	ID requested
⏵ 1	2026-02-08 17:54:08	announced

10.1021/jacsau.5c01237;

Ahn S, Kee JM, Proteomic Profiling of Oxidative Stress Response Proteins with a Methionine Sulfoxide-Inspired Activity-Based Probe. JACS Au, 6(1):310-318(2026) [pubmed]

submitter keyword: Ecoli,DJ-1,MethionineSulfoxideReductase,SO-acetylene,CysteineABPP

Jung-Min Kee
contact affiliation	Department of Chemistry, Ulsan National Institute of Science and Technology
contact email	jmkee@unist.ac.kr
lab head
Seungmin Ahn
contact affiliation	Ulsan National Institute of Science and Technology (UNIST)
contact email	asm8430@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067665
     1. Label: PRIDE project
     2. Name: Proteomic Profiling of Oxidative Stress Response Proteins Using Methionine Sulfoxide-Inspired ABPP Probes