PXD067617

PXD067617 is an original dataset announced via ProteomeXchange.

Title	Identifying Ceramide Protein Interactions using PISA
Description	Ceramides are potent influencers of cell fate and function; however, their direct molecular target(s) and mechanism of action are poorly understood. In this study, we treated primary proximal tubule epithelial cells with C2-ceramide (50uM) or its precursor C2-dihydroceramide (50uM) and used proteome integral solubility alteration (PISA) to identify potential ceramide-specific protein effectors.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:25:21.701.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD067617
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rebekah Nicholson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 10:41:26	ID requested
⏵ 1	2026-06-07 16:25:22	announced

10.6019/PXD067617;

10.1016/j.cmet.2025.10.006;

Nicholson RJ, Cede, ñ, o-Rosario L, Maschek JA, Lonergan T, Van Vranken JG, Kruse ARS, Stubben CJ, Wang L, Stuart D, Alcantara QA, Revelo MP, Rutter K, Pahulu M, Taloa J, Wu X, Kim J, Kim J, Hall I, Clark AJ, Parikh S, Spraggins J, Romero D, Blitzer JT, Gygi SP, Rutter J, Holland WL, Ramkumar N, Summers SA, Therapeutic remodeling of the ceramide backbone prevents kidney injury. Cell Metab, 38(1):135-156.e10(2026) [pubmed]

submitter keyword: PTEC, Mouse, PISA

Scott Summers
contact affiliation	Department of Nutrition and Integrative Physiology, College of Health, University of Utah, UT 84112, USA Diabetes and Metabolism Research Center, University of Utah, UT 84112, USA
contact email	Scott.a.summers@health.utah.edu
lab head
Rebekah Nicholson
contact affiliation	University of Utah
contact email	rebekah.nicholson@utah.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD067617

PRIDE project URI

• PRIDE
  1. PXD067617
     1. Label: PRIDE project
     2. Name: Identifying Ceramide Protein Interactions using PISA