PXD067615

PXD067615 is an original dataset announced via ProteomeXchange.

Title	Human RNA ligase 1 as a novel regulator of ribosome function and translation under oxidative stress
Description	Human RNA ligase 1 (Rlig1) is a recently identified human 5’-3’ RNA ligase essential for maintaining 28S rRNA integrity and promoting cell survival under oxidative stress. Although the enzymatic activity of Rlig1 implies a role in RNA maintenance or repair, its broader molecular context remains insufficiently characterised. Here we identify cellular interactors of Rlig1 using affinity enrichment coupled to mass spectrometry in a HEK293 Rlig1-KO model. Our approach revealed several RNA and DNA surveillance, degradation and repair proteins as well as a diverse set of RNA binding and RNA processing enzymes as potential interactors. Notably, ribosomal proteins were highly enriched, suggesting a link between Rlig1 and the translational machinery. Cross-linking coupled to mass spectrometry confirmed that Rlig1 interacts with 80S ribosomes in vitro and preferentially binds the large ribosomal subunit near the 28S rRNA. Polysome profiling corroborated this interaction, as Rlig1 was recruited to ribosomal fractions containing the large ribosomal subunit in response to oxidative stress. Moreover, Rlig1-deficient cells exhibited earlier polysome loss and significantly impaired translational activity compared to their WT counterparts under these conditions. These results highlight a potential novel role for Rlig1 in safeguarding translation during oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-25_01:47:27.271.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	adenylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 10:05:47	ID requested
⏵ 1	2026-05-25 01:47:27	announced

Dataset with its publication pending

submitter keyword: ribosome, XL-MS, RNA repair,Rlig1

Florian Stengel
contact affiliation	Department of Biology University of Konstanz Universitätsstrasse 10 78457 Konstanz Germany
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067615

PRIDE project URI

• PRIDE
  1. PXD067615
     1. Label: PRIDE project
     2. Name: Human RNA ligase 1 as a novel regulator of ribosome function and translation under oxidative stress