PXD067609

PXD067609 is an original dataset announced via ProteomeXchange.

Title	Ancestral and divergent roles of the Marchantia polymorpha ATAXIA TELANGIECTASIA AND RAD3-RELATED kinase in maintaining genome integrity and clonal fitness
Description	The eukaryotic ATAXIA TELANGIECTASIA AND RAD3-RELATED (ATR) kinase is essential for ensuring genomic integrity under conditions that cause replication defects. It triggers a transient cell cycle arrest and DNA repair by activating the DNA damage response pathway. While ATR kinases appear to be functionally conserved, species-specific phenotypes can be observed across the plant kingdom. To investigate the ancestral roles of the plant ATR, we identified its homolog in the model liverwort Marchantia polymorpha, which we named MpATR. Similar to other plant species, MpATR preserves meristematic cells during replication stress, whereas it mediates the response to double-strand breaks redundantly with ATAXIA-TELANGIECTASIA MUTATED (MpATM). However, unlike its counterpart in vascular plants, MpATR activity in liverwort is also essential for repressing the proliferation of differentiated cells. Furthermore, M. polymorpha does not rely on MpATR-controlled stem cell death to eliminate malignant cells. Moreover, even under control growth conditions, Mpatr mutants exhibit increasingly severe growth defects across successive vegetative clonal generations, which are aggravated in the Mpatm/atr double mutant, underscoring the critical role of MpATR in sustaining normal growth over successive generations. Overall, our study sheds light on the differential ATR dependency in liverwort versus vascular plants, emphasizing its evolutionary adaptation across plant species.
HostingRepository	PRIDE
AnnounceDate	2026-07-03
AnnouncementXML	Submission_2026-07-03_06:17:09.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shao-Li Yang
SpeciesList	scientific name: Marchantia polymorpha; NCBI TaxID: NEWT:3197;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 08:35:50	ID requested
⏵ 1	2026-07-03 06:17:09	announced

10.1093/PLCELL/KOAG151;

10.1093/plcell/koag151;

submitter keyword: Marchantia, genome integrity,DNA damage response, double strand breaks, ATR kinase, replication stress

Lieven De Veylder
contact affiliation	VIB-UGent Center for Plant Systems Biology
contact email	lieven.deveylder@psb.vib-ugent.be
lab head
Shao-Li Yang
contact affiliation	VIB-UGent Center for Plant Systems Biology
contact email	shaoli.yang@ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067609
     1. Label: PRIDE project
     2. Name: Ancestral and divergent roles of the Marchantia polymorpha ATAXIA TELANGIECTASIA AND RAD3-RELATED kinase in maintaining genome integrity and clonal fitness