PXD067606

PXD067606 is an original dataset announced via ProteomeXchange.

Title	Identification of 60S pre-rRNA binding sites of human GNL3L GTPase
Description	We reveal that the GNL3 paralogue GTPase, GNL3L, associates with pre-ribosomes. We map RNA contact sites on GNL3L as well as GNL3L binding sites on pre-rRNAs. Lack of GNL3L impairs synthesis of the 60S rRNAs and expression of GTPase inactive GNL3L causes defects in early steps of pre-rRNA processing. Impaired GTP hydrolysis by GNL3L leads to its accumulation on pre-60S particles, together with other assembly factors with proximal binding sites.
HostingRepository	PRIDE
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_06:42:53.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergei Moshkovskii
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-22 07:23:08	ID requested
⏵ 1	2026-03-05 06:42:55	announced

Thom, é CC, Lemus-Diaz N, Bloch von Blottnitz KI, Tagn, è, res S, Klein Helmkamp M, Honemann-Capito M, Hackert P, Moshkovskii S, Lenz C, Bohnsack MT, Urlaub H, Bohnsack KE, Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L. Nucleic Acids Res, 54(5):(2026) [pubmed]

10.1093/nar/gkag154;

submitter keyword: Human, GTPase, GNL3L, 60s rRNA, RNA-protein crosslinking

Henning Urlaub
contact affiliation	Research Group Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences, Germany. Bioanalytics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany.
contact email	henning.urlaub@mpinat.mpg.de
lab head
Sergei Moshkovskii
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	sergey.moshkovskiy@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD067606

PRIDE project URI

• PRIDE
  1. PXD067606
     1. Label: PRIDE project
     2. Name: Identification of 60S pre-rRNA binding sites of human GNL3L GTPase