⮝ Full datasets listing
PXD067596
PXD067596 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lauroylation-assisted workflow for both PICS and N-terminomics |
| Description | (1) Lauroylation-assisted workflow for PICS using E. coli proteome-derived tryptic peptide libraries which were blocked (succinylation or dimethylation). (2) Lauroylation-assisted N-terminomics to profile protein N-termini, ORF and non-ORF protein N-termini, using etoposide-treated cholangiocarcinoma cells by exploiting negative selection. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-05-01 |
| AnnouncementXML | Submission_2026-04-30_18:49:51.555.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD067596 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Hiroki Okanishi |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl; 4x(1)H,4x(12)C-labeled N6-succinyl-L-lysine; alpha-amino succinylated residue; unknown modification; unknown modification; N6,N6-dimethyl-L-lysine; alpha-amino dimethylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-22 01:42:58 | ID requested | |
| ⏵ 1 | 2026-04-30 18:49:52 | announced |
Publication List
| Okanishi H, Uno I, Nishimura SI, Kanai Y, Masui R, A Sensitive, Specific, and Cost-Effective Lauroylation-Assisted Workflow for Profiling Peptide-Level Protease Specificity Using Proteomic Identification of Cleavage Sites (PICS), with Applicability to Protein-Level N-Terminomics. J Proteome Res, 25(4):1878-1891(2026) [pubmed] |
Keyword List
| submitter keyword: Lauroylation, Protease, Protein N-termini, Cleavage site |
Contact List
| Ryoji Masui, Yoshikatsu Kanai | |
|---|---|
| lab head | |
| Hiroki Okanishi | |
| contact affiliation | Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004025/ |
