PXD067588

PXD067588 is an original dataset announced via ProteomeXchange.

Title	Proteomic insights into the pathogenesis of intracerebral hemorrhage: The role of blend sign in hematoma expansion
Description	The blend sign on CT imaging is a key predictor of hematoma growth in intracerebral hemorrhage (ICH), yet the underlying pathological mechanisms remain poorly understood. To address this, hematoma specimens from nine ICH patients with blend signs were collected via stereotactic minimally invasive puncture, targeting both high-density and low-density bleeding regions. Proteins were extracted and analyzed using isotope-labeled iTRAQ-based quantitative proteomics combined with LC-MS/MS. Bioinformatic analysis identified differentially expressed proteins, associated pathways, and regulatory microRNAs, followed by validation with Western blotting and ELISA. A total of 72 differentially expressed proteins were identified, with enrichment in processes including inflammation, apoptosis, oxidative stress, and metabolism. Key molecules such as cytochrome C, growth-associated protein 43, and tau were significantly upregulated in high-density regions. Integration with microRNA prediction suggested regulatory interactions, and drug screening highlighted potential therapeutic candidates such as dexketoprofen and 2-mercaptoethanesulfonic acid. This dataset provides raw mass spectrometry files and processed quantification results, offering a valuable resource for exploring the molecular basis of hematoma expansion and for identifying potential biomarkers and therapeutic targets in ICH.
HostingRepository	iProX
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_18:50:26.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hang Hang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 18:50:08	ID requested
⏵ 1	2025-08-21 18:50:27	announced

Dataset with its publication pending

submitter keyword: Intracerebral hemorrhage, hematoma, blend sign, proteomic analysis

Heng Zhao
contact affiliation	Capital Medical University
contact email	hengzhao@ccmu.edu.cn
lab head
Hang Hang
contact affiliation	The Affiliated Hospital of Guizhou Medical University
contact email	amandahh@163.com
dataset submitter

iProX dataset URI