PXD067583

PXD067583 is an original dataset announced via ProteomeXchange.

Title	Human Lyme Neuroborreliosis LC-MS (plasma)
Description	Lyme neuroborreliosis (LNB) is a nervous system infection caused by tick-borne spirochetes of the Borrelia burgdorferi sensu lato complex and is one of the most frequent bacterial infections of the nervous system in Europe. Early diagnosis remains challenging due to limited sensitivity and specificity of current tests and the need for invasive lumbar punctures, highlighting the need for improved and potentially less invasive diagnostic tools. In this study, we used bottom-up proteomics to analyze 308 cerebrospinal fluid (CSF) samples and 207 plasma samples from well-characterized cohorts, including patients with LNB, viral meningitis, other manifestations of Lyme borreliosis without central nervous system involvement, and controls with normal CSF findings. We identified diagnostic panels of regulated proteins and evaluated their performance using machine learning–based classifiers. The resulting models distinguished LNB from viral meningitis and controls in CSF with areas under the ROC curve (AUC) of 0.92 and 0.90, respectively. In plasma, LNB was distinguished from controls with an AUC of 0.80. These findings suggest a potential diagnostic role for proteomics-based biomarker panels in LNB and provide a large-scale, publicly available proteomic resource for further biomarker discovery and validation studies. NOTE the CSF search and raw files can be found in PXD067474.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_12:48:24.909.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annelaura Bach Nielsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 12:45:59	ID requested
⏵ 1	2025-09-30 12:48:25	announced

Dataset with its publication pending

submitter keyword: Human, LC-MS, Plasma, Borreliosis, LNB

Nicolai J. Wewer Albrechtsen
contact affiliation	Copenhagen University Hospital - Bispebjerg and Frederiksberg Hospital, Copenhagen, Denmark
contact email	nicolai.albrechtsen@regionh.dk
lab head
Annelaura Bach Nielsen
contact affiliation	University of Copenhagen
contact email	annelaura@live.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD067583

PRIDE project URI

• PRIDE
  1. PXD067583
     1. Label: PRIDE project
     2. Name: Human Lyme Neuroborreliosis LC-MS (plasma)