PXD067579

PXD067579 is an original dataset announced via ProteomeXchange.

Title	Effects of Mos-Kinase inhibition on protein levels during meiosis of starfish oocytes
Description	The development of a haploid, fertilizable female gamete involves specialized meiotic divisions of the fully grown oocyte. An essential feature of oocyte meiosis is the reduction of genomic content to half, which is achieved by two successive divisions without an intervening S-phase. A known regulator of this event is a kinase called Mos that is exclusively expressed in oocytes during meiosis and activates the MEK/ERK/MAPK pathway. However, the downstream targets of the Mos-MAPK pathway and their significance in oocyte meiosis remains largely understudied. In this project, using phosphoproteomics on starfish (Patiria miniata) oocytes, we identify two predominant subsets of proteins that are targeted by Mos-MAPK at the critical time point - metaphase of meiosis I. These include proteins involved in CPE-mediated polyadenylation and cytoskeleton regulators. To complement this experiment, we performed DIA proteomics on Mos inhibited (20 micromolar U0126) and control oocytes at subsequent stages of meiosis (at 36 mins, 60 minutes and 90 minutes post hormone addition in U0126 inhibited oocytes and at metaphase I, meiosis I to meiosis II transition, metaphase II, 2 pronuclei and finally at the first mitotic division of the zygote for control oocytes). In this experiment, we identified 7,480 protein groups of which 5,103 protein groups could be quantified. We did not find significant changes in protein abundance across these time points and conditions, indicating that the proteome of starfish oocytes remains largely stable during meiosis.
HostingRepository	MassIVE
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_04:53:30.962.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olexandr Dybkov
SpeciesList	scientific name: Patiria miniata;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 10:49:23	ID requested
⏵ 1	2025-09-03 04:53:31	announced

Ivan Avilov, Yehor Horokhovskyi, Pooja Mehta, Luisa Welp, Jasmin Jakobi, Mingfang Cai, Aleksander Orzechowski, Henning Urlaub, Juliane Liepe and Peter Lenart. Phosphoproteomic identification of Mos-MAPK targets in meiotic cell cycle and asymmetric division. doi: 10.1083/jcb.202312140.

submitter keyword: meiosis, cytoskeleton, translational regulation, oocytes, Patiria miniata, DatasetType:Proteomics

Juliane Liepe
contact affiliation	Research Group Quantitative and Systems Biology, Max Planck Institute for Multidisciplinary Sciences
contact email	juliane.liepe@mpinat.mpg.de
lab head
Henning Urlaub
contact affiliation	Research Group Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences; Bioanalytics, Department of Clinical Chemistry, University Medical Center Goettingen
contact email	henning.urlaub@mpinat.mpg.de
lab head
Peter Lenart
contact affiliation	Research Group Cytoskeletal Dynamics in Oocytes, Max Planck Institute for Multidisciplinary Sciences
contact email	peter.lenart@mpinat.mpg.de
lab head
Olexandr Dybkov
contact affiliation	MPINAT
contact email	odybkov@mpinat.mpg.de
dataset submitter

MassIVE dataset URI

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