PXD067568

PXD067568 is an original dataset announced via ProteomeXchange.

Title	Fungal oxidation of short-chain gaseous alkanes under extremely acidic conditions
Description	Short-chain gaseous alkanes (SCGAs) are key constituents of natural gas. When released into the atmosphere, they can significantly impact air quality and tropospheric chemistry. Although microbes can breakdown SCGAs through aerobic oxidation, the microbial taxa involved and their metabolic traits under acidic conditions remain poorly understood. Here, we report Fonsecaea alkanivorans sp. nov., a fungal species that can mediate aerobic SCGA oxidation at extremely low pH (2.2). Our long-term bioreactor experiments in extreme acidity, batch tests, and analysis of small subunit (SSU) rRNA sequences demonstrated the capacity of this enriched fungus in oxidizing ethane, propane, and butane. Meta-omics analyses revealed expression of genes associated with SCGA metabolism, and the function of cytochrome P450 monooxygenases (from CYP52 family) in activating C–H bonds, converting alkanes to corresponding alkanols. These findings uncover a novel fungal pathway for SCGA oxidation under extreme acidity and highlight the broader role of fungi and cytochrome P450 in gaseous hydrocarbon cycling.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_07:10:26.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiawei Liu
SpeciesList	scientific name: Fonsecaea; NCBI TaxID: NEWT:40354;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 07:01:48	ID requested
⏵ 1	2026-04-09 07:10:26	announced

Dataset with its publication pending

submitter keyword: Short-chain gaseous alkane oxidation

Fungi

Fonsecaea

Cytochrome P450

Dr Jianhua Guo
contact affiliation	Australian Centre for Water and Environmental Biotechnology, The University of Queensland
contact email	j.guo1@uq.edu.au
lab head
Xiawei Liu
contact affiliation	Australian Centre for Water and Environmental Biotechnology
contact email	xiawei.liu@uq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067568
     1. Label: PRIDE project
     2. Name: Fungal oxidation of short-chain gaseous alkanes under extremely acidic conditions