PXD067564

PXD067564 is an original dataset announced via ProteomeXchange.

Title	A subset of type 4 secretion system effectors of Brucella spp. associates to outer membrane vesicles
Description	The establishment of a replicative niche in the hostile environment of the host presents an enormous challenge for pathogens. Intracellular pathogens such as Brucella spp., the Gram-negative causative agents of Brucellosis, must subvert diverse host functions to ensure survival and replication. To schieve this, Brucella translocates effector proteins into host cells via its type 4 secretion system (T4SS), a key virulence factor. Here, we demonstrate that a subset of previously described effector proteins associates with outer membrane vesicles (OMVs) in different Brucella species. Most of these effector proteins encode predicted signal peptides for periplasmic export or transmembrane domains. Among them, BspC and VceA carry functional signal peptides that direct their export into the periplasm in a Sec-dependent manner. From the periplasm these proteins are subsequently secreted into the extracellular milieu, likely via the formation and release of OMVs. Our findings provide new insights into protein secretion by Brucella, suggesting that OMVs may represent an alternative secretion pathway to the T4SS.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-05_02:33:37.526.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naadir Ganief
SpeciesList	scientific name: Brucella abortus 2038; NCBI TaxID: NEWT:1310173;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 06:34:00	ID requested
⏵ 1	2026-01-05 02:33:38	announced

10.1093/FEMSML/UQAF046;

submitter keyword: PASEF, OMVs, Shotgun,Brucella, DDA

Christoph Dehio
contact affiliation	Biozentrum, University of Basel, Basel, Switzerland
contact email	christoph.dehio@unibas.ch
lab head
Naadir Ganief
contact affiliation	Biozentrum, University of Basel
contact email	naadir.ganief@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067564

PRIDE project URI

• PRIDE
  1. PXD067564
     1. Label: PRIDE project
     2. Name: A subset of type 4 secretion system effectors of Brucella spp. associates to outer membrane vesicles