PXD067563

PXD067563 is an original dataset announced via ProteomeXchange.

Title	GYF protein-mediated FLC translational repression-coupled mRNA decay promotes flowering time in Arabidopsis
Description	Plant flowering represents a critical transition from vegetative growth to reproductive growth and serves as an important marker of plant developmental maturity. The flowering repressor gene FLC is a key regulator of floral transition. Although extensive research has been conducted on FLC, the mechanisms governing FLC mRNA degradation and translational regulation remain unclear. In previous studies, we identified the GYF family by screening regulatory factors near the nuclear pore complex (NPC). We found that GYF proteins are enriched near the nuclear membrane and can interact with components of both the NPC and processing bodies (P-bodies). Functional studies revealed that gyf mutants exhibit a late-flowering phenotype, primarily due to elevated FLC mRNA levels. In vitro and in vivo biochemical experiments demonstrated that GYF proteins directly bind to the 5' UTR and 3' UTR of FLC mRNA, cooperate with 4EHP to participate in translation-coupled mRNA degradation and translational repression of FLC.
HostingRepository	PRIDE
AnnounceDate	2026-09-08
AnnouncementXML	Submission_2026-09-07_18:42:55.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingtang Ma
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 06:20:27	ID requested
⏵ 1	2026-09-07 18:42:55	announced

Dataset with its publication pending

submitter keyword: translation inhibition, mRNA decay, FLC,GYF, flowering

Yu Tang
contact affiliation	Peking University Institute of Advanced Agricultural Sciences
contact email	yu.tang@pku-iaas.edu.cn
lab head
Yingtang Ma
contact affiliation	Peking University Institute of Advanced Agricultural Sciences
contact email	1542293361@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD067563

PRIDE project URI

• PRIDE
  1. PXD067563
     1. Label: PRIDE project
     2. Name: GYF protein-mediated FLC translational repression-coupled mRNA decay promotes flowering time in Arabidopsis