PXD067551

PXD067551 is an original dataset announced via ProteomeXchange.

Title	Bidirectional interaction between PGE₂-preconditioned mesenchymal stem cells and myofibroblasts mediates antifibrotic effects: A proteomic investigation into equine endometrial fibrosis reversal
Description	Endometrosis is a prevalent fibrotic condition in mares that impairs reproductive efficiency by inducing transdifferentiation of endometrial stromal cells into myofibroblasts, leading to excessive ECM deposition. Methods: To elucidate the molecular mechanisms underlying fibrosis resolution, this study employed comprehensive proteomic techniques, including LC-MS/MS and SILAC, to analyze the interaction between myofibroblasts and mesenchymal stem cells derived from the endometrium (ET-eMSCs) preconditioned with PGE₂. An in vitro co-culture system was used, with samples collected at baseline and after 48 hours. Results: Proteomic analysis identified significant alterations in proteins associated with ECM remodeling, immune regulation, and cellular stress response. Notably, proteins involved in collagen degradation, antioxidant defense, and growth factor signaling pathways were differentially abundant. Network analyses demonstrated robust interactions among these proteins, suggesting coordinated modulatory effects. The data indicate that PGE₂-primed ET-eMSCs induce a shift in myofibroblast secretory profiles, promoting a reduction in ECM stiffness, tissue reorganization, and activation of resolution pathways. Conclusions: These findings reinforce the therapeutic potential of mesenchymal stem cell-based interventions for fibrotic diseases of the endometrium, opening avenues for regenerative strategies to restore reproductive function in mares.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:38:46.066.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillermo Nourdin
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 05:14:08	ID requested
⏵ 1	2025-09-28 16:38:46	announced

M, é, ndez-P, é, rez L, Wong YS, Ib, á, ñ, ez BO, Martinez-Hormaza I, Rodr, í, guez-, Á, lvarez L, Castro FO, -Preconditioned Mesenchymal Stem Cells and Myofibroblasts Mediates Anti-Fibrotic Effects: A Proteomic Investigation into Equine Endometrial Fibrosis Reversal. Proteomes, 13(3):(2025) [pubmed]

10.3390/proteomes13030041;

submitter keyword: Endometrosis

Equine mesenshymal stem cells

Mare endometrial

precondition with PGE2

SILAC

myofibloblasts - Equine mesenshymal stem cells interaction

Fidel O. Castro
contact affiliation	Laboratory of Animal Biotechnology, Faculty of Veterinary Sciences, Department of Animal Science, Universidad de Concepción, Chillán, Chile.
contact email	fidcastro@udec.cl
lab head
Guillermo Nourdin
contact affiliation	MELISA Institute
contact email	gnourdin@melisainstitute.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067551
     1. Label: PRIDE project
     2. Name: Bidirectional interaction between PGE₂-preconditioned mesenchymal stem cells and myofibroblasts mediates antifibrotic effects: A proteomic investigation into equine endometrial fibrosis reversal