PXD067546

PXD067546 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry on NUP153(NG)AID cells infected with WNV
Description	The nuclear pore complex is built of multiple copies of different nucleoporins (NUP), that together enable the transport across the nuclear envelope. One of the NUPs is NUP153, which primarily is involved in the shuttling of RNA, yet also has functions in the assembly and structure of the pore. We have implemented the auxin inducible degron (AID) system to rapidly deplete NUP153 in cells and evaluate the impact this has on orthoflavivirus infection. To determine which viral and cellular proteins are impacted by NUP153 depletion we have performed mass spectrometry experiments in AID and control cells infected with West Nile virus, to help elucidate the molecular mechanism of NUP153 in orthoflavivirus infection.
HostingRepository	PRIDE
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_07:23:07.701.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Klaus Faserl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: West Nile virus; NCBI TaxID: NEWT:11082;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 05:00:02	ID requested
⏵ 1	2026-03-05 07:23:08	announced

Dataset with its publication pending

submitter keyword: West Nile virus, AID,NUP153, Orthoflavivirus

Klaus Faserl
contact affiliation	Institute of Medical Biochemistry, Protein Core Facility, Medical University of Innsbruck, Innsbruck, Austria.
contact email	klaus.faserl@i-med.ac.at
lab head
Klaus Faserl
contact affiliation	Innsbruck Medical University, Biocenter
contact email	klaus.faserl@i-med.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD067546

PRIDE project URI

• PRIDE
  1. PXD067546
     1. Label: PRIDE project
     2. Name: Mass Spectrometry on NUP153(NG)AID cells infected with WNV