PXD067538

PXD067538 is an original dataset announced via ProteomeXchange.

Title	Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4.
Description	The cell-type-specific function of transcription factors (TFs) is crucial for determining several cellular identities. It is unclear how a single TF can function specifically in different cell types. Here, we define the molecular features that enable OCT4 to reprogram somatic cells into pluripotent or trophoblast stem cells, maintain the self-renewal of embryonic stem (ES) cells, and drive lineage commitment during early embryonic development. Embedded within the intrinsically disordered regions (IDRs) of OCT4, we uncover short linear peptides that are essential for reprogramming (SLiPERs) but dispensable for ES self-renewal. SLiPERs adopt a quasi-ordered state and, during reprogramming, recruit a unique set of proteins to closed chromatin that are unnecessary for ES self-renewal. Interestingly, SLiPERs are not required during early gastrulation but are essential for embryos to develop beyond late gastrulation. Removing SLiPERs leads to aberrant OCT4 binding, derailing the regular transition of ES cells out of pluripotency. Our findings identify modules within IDRs that contribute to the functional versatility and specificity of TFs.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_07:31:53.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christos Spanos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-21 04:33:30	ID requested
⏵ 1	2025-09-30 07:31:54	announced

10.1038/S41467-025-63806-3;

submitter keyword: OCT4, intrinsically disordered regions, embryonic stem (ES) cells,Transcription Factors, SLiPERs

Dr. Abdenour Soufi
contact affiliation	University of Edinburgh
contact email	Abdenour.Soufi@ed.ac.uk
lab head
Christos Spanos
contact affiliation	University of Edinburgh
contact email	spanos.christos@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD067538

PRIDE project URI

• PRIDE
  1. PXD067538
     1. Label: PRIDE project
     2. Name: Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4.