PXD067524
PXD067524 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Site-specific Profiling of Lysine Itaconylation via a Thiol-based Probe |
| Description | Lysine itaconylation is a novel post - translational modification (PTM) that was recently discovered in macrophage proteomes mediated by the immunoregulatory metabolite, itaconate. However, there is currently an absence of comprehensive and high - accuracy analytical methods for the global identification of itaconylation sites in proteomes, which limits its biological function study. Here, we developed a thiol-based bioorthogonal probe, BMAyne, to site-specifically profile itaconylation in macrophage proteomes by a quantitative chemoproteomic strategy. Notably, 29 endogenous itaconylated proteins were identified in lipopolysaccharide (LPS)-stimulated macrophages using BMAyne, which complemented the results obtained by promiscuous antibody enrichment. Our effort provides a unique chemical tool to enrich endogenous lysine itaconylation and establishes a rich database for guiding subsequent functional studies of this unique PTM. |
| HostingRepository | iProX |
| AnnounceDate | 2025-08-20 |
| AnnouncementXML | Submission_2025-08-20_18:41:51.242.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiaping Fu |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse; Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-20 18:41:27 | ID requested | |
| ⏵ 1 | 2025-08-20 18:41:51 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Protein itaconylation |
| Thiol-based probe |
| Chemoproteomics |
Contact List
| Chu Wang | |
|---|---|
| contact affiliation | Department of Chemical Biology, College of Chemistry & Molecular Engineering (CCME), Peking University |
| contact email | chuwang@pku.edu.cn |
| lab head | |
| Xiaping Fu | |
| contact affiliation | Department of Chemical Biology, College of Chemistry & Molecular Engineering (CCME), Peking University |
| contact email | xiapingfu@pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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