⮝ Full datasets listing
PXD067497
PXD067497 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In vivo Identification of Arabidopsis SIZ1 SUMO E3 Ligase Substrates by TurboID-Mediated Proximity Labeling |
| Description | To investigate how SIZ1 promotes immune activation, we employed an enzyme-catalyzed proximity-labeling approach to identify its in vivo substrates. We fused SIZ1 with TurboID, an engineered promiscuous biotin ligase, and generated stable transgenic Arabidopsis expressing pSIZ1::SIZ1g-TurboID-HA. Ten-day-old pSIZ1::SIZ1-TurboID-HA and WT plants were treated with free biotin for 5 hours to achieve effective proximity labeling, after which labeled proteins were affinity purified and identified by label-free quantitative mass spectrometry (LFQMS) with two biological replicates. |
| HostingRepository | iProX |
| AnnounceDate | 2025-08-19 |
| AnnouncementXML | Submission_2026-02-27_23:16:16.367.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xuanyi Chen |
| SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-19 00:10:38 | ID requested | |
| ⏵ 1 | 2026-02-27 23:16:16 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Arabidopsis thaliana, seedling, KA120, SIZ1, TurboID |
Contact List
| Yangnan Gu | |
|---|---|
| contact affiliation | Department of Plant & Microbial Biology, University of California, Berkeley |
| contact email | guyangnan@berkeley.edu |
| lab head | |
| Xuanyi Chen | |
| contact affiliation | Northwest A&F University |
| contact email | chenxuanyi@nwafu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
