PXD067483

PXD067483 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of HT-1080 cells exposed to single-atom engineered graphene derivatives versus copper ionophore elesclomol and glutathione peroxidase 4 inhibitor RSL3
Description	This dataset presents a comparative proteomic analysis of the human fibrosarcoma cell line HT-1080 in response to treatment with nitrogen-doped graphene acid (NGA) and the single atom engineered derivatives, NGA-Fe3+ and NGA-Cu2+. To further investigate the role of metal-induced cell death pathways, cells were exposed to elesclomol, a well-known copper ionophore, and RSL3, a ferroptosis activating compound. The main objective was to characterize the proteomic alterations associated with single atom engineered NGA to elucidate potential mechanisms of action. We also examined the effects of elesclomol and RSL3 to provide reference datasets for copper-induced cytotoxicity (aka cuproptosis) and ferroptosis, respectively. This dataset provides insights into the cellular responses triggered by NGA and its derivatives and how these materials impinge on regulated cell death pathways.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_18:37:28.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Massimiliano Gaetani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-18 11:02:47	ID requested
⏵ 1	2026-02-01 18:37:29	announced

Sae-Fung A, Fadeel B, Copper ionophore elicits calpain-dependent paraptosis coincident with proteotoxic stress. Cell Commun Signal, 24(1):47(2025) [pubmed]

10.1186/s12964-025-02558-5;

submitter keyword: graphene, expression proteomics, ferroptosis, single atom engineering,cuproptosis, elesclomol, fibrosarcoma

Massimiliano Gaetani
contact affiliation	Chemical Proteomics, Division of Chemistry I, Department of Medical Biochemistry and Biophysiscs (MBB), Karolinska Institutet, Stockholm, Sweden
contact email	massimiliano.gaetani@ki.se
lab head
Massimiliano Gaetani
contact affiliation	Karolinska Institutet
contact email	massimiliano.gaetani@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD067483

PRIDE project URI

• PRIDE
  1. PXD067483
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of HT-1080 cells exposed to single-atom engineered graphene derivatives versus copper ionophore elesclomol and glutathione peroxidase 4 inhibitor RSL3