⮝ Full datasets listing
PXD067479
PXD067479 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deletion of Murine Endothelial Bag3 Alters the Cellular Proteome Among Organs of Origin |
| Description | BAG3 (Bcl-2 associated athanogene 3) is a multifunctional protein with pleiotropic effects in multiple cell types. Despite our knowledge of its role in cardiovascular disease, it’s specific role in endothelial cells (ECs) is unknown. The purpose of this study was to identify differences in the EC proteome of multiple tissues before and after cell specific deletion of Bag3. We hypothesized that loss would alter the baseline proteome of ECs uniquely in each tissue. Cdh5(PAC)-CreERT2;Bag3f/f mice received tamoxifen (KO; n=18) or vehicle (WT; n=18) and tissues (brain, heart, lung, and peripheral skeletal muscle-skm) were collected for FACS sorting of ECs from equal numbers of male and female mice at >22 weeks of age, followed by LC-MS/MS label-free proteomics. Initial comparisons of the WT proteomes between tissues revealed differential abundance of EC proteins (p<0.05), including: 379 brain v heart; 325 brain v lung; 501 brain v skm; 354 heart v lung; 107 heart v skm; and 442 lung v skm. Additionally, the EC mitochondrial proteome was unique to each tissue of origin, with significantly (p<0.05) higher proportions dedicated to complexes I, III, IV and V, in heart compared to the other tissues. In brain EC (adj p<0.1; >1.15-fold), KO increased 45 proteins (Eif1ax L2FC=3.54; Arl15 L2FC=3.52) and decreased 16 (Ca1 L2FC=-2.44; Hbb-y L2FC=-2.29). In heart ECs, KO increased 33 (H3c1 L2FC=1.39; Pfn1 L2FC=1.29) and decreased 7 (Hal L2FC=-1.41; Skt L2FC=-0.66) proteins. In lung ECs, 34 proteins increased (Snrpf L2FC=3.48; Tmem30a L2FC=1.14) and 30 decreased (Prkar1a L2FC=-1.07; Psmd14 L2FC=-0.963). KO demonstrated the largest effect on skm ECs, increasing 30 proteins (Mybpc2 L2FC=4.81; PFKM L2FC=3.02) and decreasing 65 (CDC42 L2FC=-2.44; Prpf8 L2FC=-2.24). Overall, these results demonstrate that the EC proteome of different tissues is unique and that the loss of BAG3 in these cells differentially alters a small proportion of the EC specific proteome. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-08-19 |
| AnnouncementXML | Submission_2026-08-18_08:00:04.744.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kelsey Fisher-Wellman |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-18 09:53:11 | ID requested | |
| ⏵ 1 | 2026-08-18 08:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: mouse, BAG3, endothelium |
Contact List
| Joseph M. McClung | |
|---|---|
| lab head | |
| Kelsey Fisher-Wellman | |
| contact affiliation | Wake Forest University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004017/ |
