PXD067464

PXD067464 is an original dataset announced via ProteomeXchange.

Title	Proteomic Dataset of Non-Specific Interactors in Leishmania infantum
Description	This dataset provides a proteomic resource of non-specific protein interactions in Leishmania infantum identified by affinity purification coupled to mass spectrometry (AP-MS/MS). Protein extracts from promastigotes expressing Cas9/T7 RNA polymerase were subjected to immunoprecipitation using HA, myc, and His affinity systems, followed by LC–MS/MS analysis. Raw spectral data were processed using MaxQuant against the L. infantum reference proteome from UniProtKB. The dataset includes raw mass spectrometry files, peptide and protein identification tables, and label-free quantification (LFQ) data, all publicly available via the ProteomeXchange Consortium under the identifier PXD067464. After data filtering, a total of 566 unique proteins were identified across all conditions, including 60 proteins consistently detected in all three affinity systems, representing putative non-specific binders. Among these, metabolism-related proteins (30%) and ribosomal components (28%) were the most abundant functional classes. This dataset provides a valuable reference for background protein binding in AP-MS/MS experiments in Leishmania , supporting improved interpretation of interactome studies and benchmarking of affinity purification strategies.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_09:02:19.408.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wesley Regatieri
SpeciesList	scientific name: Leishmania infantum JPCM5; NCBI TaxID: NEWT:435258;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-18 06:11:44	ID requested
⏵ 1	2026-05-20 09:02:19	announced

10.1016/J.DIB.2026.112840;

submitter keyword: None

Felipe Roberti
contact affiliation	Department of Genetics and Evolution, Federal University of São Carlos, São Paulo, Brazil
contact email	frt@ufscar.br
lab head
Wesley Regatieri
contact affiliation	Federal University of São Carlos
contact email	wesley.klaysson@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD067464

PRIDE project URI

• PRIDE
  1. PXD067464
     1. Label: PRIDE project
     2. Name: Proteomic Dataset of Non-Specific Interactors in Leishmania infantum