PXD067405

PXD067405 is an original dataset announced via ProteomeXchange.

Title	NAE1/UBA3-UBE2M are E1 and E2 Enzymes for the URM1 Modification in Human Cells
Description	The Urm1 protein, a member of the ubiquitin (Ub)-related modifier family, is highly conserved across evolution and plays a crucial role as a sulfur donor in tRNA thiolation. Despite its importance, Urm1 remains relatively understudied in mammalian eukaryotes, leading to numerous unknown functions. As of now, no deubiquitinases that interact with Urm1 in mammalian cells have been reported, nor are there known conjugating enzymes (E2), or ligases (E3) involved in the Urm1 cascade. Our study introduces an activity-based Urm1 probe featuring a C-terminal alkyne group, which specifically targets the active-site cysteine residues of deubiquitinases and other key enzymes in the degradation pathway. Using a proteomic approach, we identified deubiquitinases, E1, and E2 enzyme that bind with Urm1 in mammalian cells, paving the way for further exploration and understanding of Urm1's functional interactions.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-23_15:42:13.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhongwen Cao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-15 19:02:04	ID requested
⏵ 1	2026-03-23 15:42:13	announced

Dataset with its publication pending

submitter keyword: E1/E2 enzymes LC-MS/MS

Wenshe Liu
contact affiliation	Texas A&M University
contact email	wliu@chem.tamu.edu
lab head
Zhongwen Cao
contact affiliation	University of California, Riverside
contact email	zcao037@ucr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067405
     1. Label: PRIDE project
     2. Name: NAE1/UBA3-UBE2M are E1 and E2 Enzymes for the URM1 Modification in Human Cells