PXD067401

PXD067401 is an original dataset announced via ProteomeXchange.

Title	Proteomic Comparisoadsfasn of Intact and Fetoscopy-Induced Fetal Membrane Sites
Description	Iatrogenic preterm premature rupture of fetal membranes (iPPROM) following fetoscopic interventions remains a major barrier to the advancement of fetal therapies. The mechanisms underlying iPPROM are poorly understood, but the inability of fetal membrane (FM) defects to heal spontaneously likely plays a key role, contrasting with the regenerative potential of amniotic membranes in other contexts. To assess the impact of fetoscopic procedures on FMs, tissue samples from patients who underwent laser surgery for twin-to-twin transfusion syndrome (16–27 weeks gestation, n=8) were collected after cesarean delivery at 29–35 weeks. Samples were categorized by proximity to the trocar site and analyzed using proteomic and histological methods. While differential expression analysis in the amnion revealed no significant changes, pathway enrichment indicated increased collagen deposition at defect sites. In the chorion, sevendifferentially expressed proteins were identified, largely linked to enhanced intercellular contact stability. These findings suggest the amnion may respond to mechanical stress by reinforcing structural integrity through collagen deposition, while the chorion may attempt to stabilize cell junctions. However, no other signs of tissue regeneration were observed. This study provides molecular and cellular evidence that FMs lacks a substantial healing response post-surgery, underscoring the need for biologically informed repair strategies.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-05_03:48:08.283.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lukas Moser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-15 15:16:54	ID requested
⏵ 1	2026-01-05 03:48:08	announced

10.1038/S41390-025-04692-9;

submitter keyword: fetal membrane, TTTS, human, iPPROM

Martin Ehrbar
contact affiliation	University of Zurich, University Hospital of Zurich
contact email	martin.ehrbar@usz.ch
lab head
Lukas Moser
contact affiliation	University of Zurich, University Hospital Zurich
contact email	lukas.moser@usz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD067401

PRIDE project URI

• PRIDE
  1. PXD067401
     1. Label: PRIDE project
     2. Name: Proteomic Comparisoadsfasn of Intact and Fetoscopy-Induced Fetal Membrane Sites