PXD067397

PXD067397 is an original dataset announced via ProteomeXchange.

Title	Identification and characterization of the zebra finch (Taeniopygia guttata) sperm proteome
Description	Spermatozoa exhibit remarkable variability in size, shape, and performance. Our understanding of the molecular basis of this variation, however, is limited, especially in avian taxa. The zebra finch (Taeniopygia guttata) is a model organism in the study of avian sperm biology and sperm competition. Using LC-MS based proteomics, we identify and describe 494 proteins of the zebra finch sperm proteome (ZfSP). Gene ontology and associated bioinformatics analyses revealed a rich repertoire of proteins essential to sperm structure and function, including proteins linked to metabolism and energetics, as well as tubulin binding and microtubule related functions. The ZfSP also contained a number of immunity and defense proteins and proteins linked to sperm motility and sperm-egg interactions. Additionally, while most proteins in the ZfSP appear to be evolutionarily constrained, a small subset of proteins are evolving rapidly. Finally, in a comparison with the sperm proteome of the domestic chicken, we found an enrichment of proteins linked to catalytic activity and cytoskeleton related processes. As the first described passerine sperm proteome, and one of only two characterized avian sperm proteomes, the ZfSP provides a significant step towards a platform for studies of the molecular basis of sperm function and evolution in birds.
HostingRepository	PRIDE
AnnounceDate	2025-09-16
AnnouncementXML	Submission_2025-09-16_13:11:50.771.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yishai Gilron
SpeciesList	scientific name: Taeniopygia guttata (Zebra finch) (Poephila guttata); NCBI TaxID: 59729;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-15 13:54:21	ID requested
⏵ 1	2025-09-16 13:11:51	announced

10.1016/j.jprot.2018.10.009;

Rowe M, Skerget S, Rosenow MA, Karr TL, Identification and characterization of the zebra finch (Taeniopygia guttata) sperm proteome. J Proteomics, 193():192-204(2019) [pubmed]

submitter keyword: Avian sperm Passerines Sexual reproduction Sperm proteins Positive selection

Timothy Karr
contact affiliation	Research Associate Professor, Biosciences Arizona State University(ASU) Neurodegenerative Disease Associate Faculty Neurodegenerative Disease Research Center Associate Faculty Team ASU-Banner Neurodegenerative Disease Research Center
contact email	tkarr@asu.edu
lab head
Yishai Gilron
contact affiliation	Staff scientist
contact email	ygilron@asu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD067397

PRIDE project URI

• PRIDE
  1. PXD067397
     1. Label: PRIDE project
     2. Name: Identification and characterization of the zebra finch (Taeniopygia guttata) sperm proteome