PXD067383

PXD067383 is an original dataset announced via ProteomeXchange.

Title	The C-terminal SUMOylation of αKNL2 facilitates its centromeric localization and interaction with CENH3
Description	The centromere is a specialized domain that facilitates chromosome segregation during mitosis and serves as the site for kinetochore formation. KINETOCHORE NULL2 (αKNL2) is essential for the recognition and loading of the centromeric histone H3 variant, CENH3, to centromeres. A yeast two-hybrid screen for αKNL2 interactors identified components of the SUMOylation pathway. However, the role of αKNL2 SUMOylation in Arabidopsis has not yet been determined. Bioinformatic and functional analysis identified three SUMOylation and two SUMO-interacting motif (SIM) sites in the C-terminal region of αKNL2, which are critical for growth, fertility, and chromosome alignment. Of the three SUMOylation sites, Lys474 was the most critical for the centromeric localization of αKNL2, underscoring the importance of αKNL2 SUMOylation for its function. Additionally, both in vitro and in vivo assays showed that αKNL2-C undergoes SUMOylation by SUMO1 or SUMO3. The MS analysis revealed that SUMO1 modified several lysine residues, including K424, K445, K511, K540, K572, K583, K585, and K598, indicating extensive SUMOylation across the C-terminal region. In contrast, SUMO3-dependent modification was detected only at K424, K511, and K598. These findings are consistent with our in vitro assay results, which showed stronger SUMOylation by the SUMO1 isoform than SUMO3.
HostingRepository	PRIDE
AnnounceDate	2025-11-19
AnnouncementXML	Submission_2025-11-19_14:54:44.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	sumoylated lysine
Instrument	timsTOF Pro; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-15 01:57:15	ID requested
⏵ 1	2025-11-19 14:54:44	announced

10.1016/J.XPLC.2025.101617;

submitter keyword: Arabidopsis thaliana, SUMOylation,alphaKNL2

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD067383

PRIDE project URI

• PRIDE
  1. PXD067383
     1. Label: PRIDE project
     2. Name: The C-terminal SUMOylation of αKNL2 facilitates its centromeric localization and interaction with CENH3