PXD067354

PXD067354 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling – LC/MS-MS with ERC1-TurboID/atg7-2 Arabidopsis seedlings
Description	Given that ERC1 proteins are enriched on membraneless condensates together with ATG8 proteins prior to autophagosome formation, we hypothesized that ERC1-positive condensates might serve as an assembly site for ATG8 proteins and other adaptors before ATG8 proteins are conjugated onto the phagophore membrane. In order to identify other possible components related to the ERC1 condensates, we generated transgenic plants expressing TurboID-tagged ERC1 in the atg7-2 background. TurboID-coupled mass spectrometry revealed that, in addition to ATG8e, the selective autophagy receptor NBR1 was also significantly enriched.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_11:50:36.893.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-14 12:23:28	ID requested
⏵ 1	2025-10-07 11:50:37	announced

10.1101/2024.09.09.611939;

Chung KK, Zhao Z, Law KC, Ma J, Chiang CH, Leung KH, Shrestha R, Wu Y, Li C, Lee KM, Feng L, Li X, Wong KB, Xu SL, Gao C, Zhuang X, Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance. bioRxiv, ():(2024) [pubmed]

submitter keyword: ERC protein family, ATG8, TurboID, Biomolecular condensation, Autophagosome formation

Shouling Xu
contact affiliation	Carnegie Mass Spec Facility
contact email	sxu@ciw.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD067354

PRIDE project URI

• PRIDE
  1. PXD067354
     1. Label: PRIDE project
     2. Name: Proximity labeling – LC/MS-MS with ERC1-TurboID/atg7-2 Arabidopsis seedlings